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Helping to create the lysosome is a dangerous job—stick with your task for too long and you might end up as dinner. Seaman (page 111) and Arighi et al. (page 123) now describe how the cation-independent mannose 6-phosphate receptor (CI-MPR) escorts lysosomal enzymes toward their future home but then escapes just in time thanks to a complex of proteins called the retromer.
Without retromer, CI-MPR (red) leaks through to late endosomes and lysosomes (green).
The retromer was first characterized in yeast, where it drags Vps10p from endosomes back to the Golgi. Vps10p and the mammalian CI-MPR have no sequence homology but do perform similar functions. So the researchers tested whether the retromer could also rescue CI-MPR.
They first confirmed that both CI-MPR and the mammalian retromer are located in endosomes, with additional CI-MPR in the trans-Golgi network. After either knockout or...
The Rockefeller University Press
2004
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