page 341, Babu et al. provide a possible explanation for this apparent contradiction and describe new mouse models that should be useful in studying cancer progression.The authors disrupted two highly homologous mouse genes, encoding the nuclear transport factor Rae1 and the checkpoint protein Bub3. Homozygous null mutations in either Rae1 or Bub3 are embryonic lethal. Heterozygous mice with only one copy of Rae1 survive, but exhibit mitotic checkpoint defects and chromosome mis-segregation, and are predisposed to carcinogen-induced lung cancer. Heterozygous Bub3 knockout mice have a strikingly similar phenotype. Surprisingly, overexpressing Rae1 in the mice compensates for haploinsufficiency of either Rae1 or Bub3, indicating significant overlap in the two proteins' functions.
The results show that the mammalian mitotic checkpoint system is extremely sensitive to underexpression of its components. Epigenetic effects in tumor cells might start a vicious cycle, in which down-regulation of a checkpoint protein causes chromosome loss, leading to the loss of other checkpoint proteins and further chromosome missegregation. The new strains can now be crossed with existing mouse cancer models to study aneuploidy in tumor pathogenesis. ▪