An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti–axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.
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15 May 2000
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May 15 2000
A Direct Interaction of Axonin-1 with Ngcam-Related Cell Adhesion Molecule (Nrcam) Results in Guidance, but Not Growth of Commissural Axons
Dora Fitzli,
Dora Fitzli
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
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Esther T. Stoeckli,
Esther T. Stoeckli
bDepartment of Integrative Biology, University of Basel, CH-4051 Basel, Switzerland
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Stefan Kunz,
Stefan Kunz
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
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Kingsley Siribour,
Kingsley Siribour
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
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Christoph Rader,
Christoph Rader
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
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Beat Kunz,
Beat Kunz
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
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Serguei V. Kozlov,
Serguei V. Kozlov
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
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Andrea Buchstaller,
Andrea Buchstaller
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
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Robert P. Lane,
Robert P. Lane
cDivision of Biology 156-29, California Institute of Technology, Pasadena, California 91125
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Daniel M. Suter,
Daniel M. Suter
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
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William J. Dreyer,
William J. Dreyer
cDivision of Biology 156-29, California Institute of Technology, Pasadena, California 91125
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Peter Sonderegger
Peter Sonderegger
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
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Dora Fitzli
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
Esther T. Stoeckli
bDepartment of Integrative Biology, University of Basel, CH-4051 Basel, Switzerland
Stefan Kunz
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
Kingsley Siribour
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
Christoph Rader
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
Beat Kunz
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
Serguei V. Kozlov
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
Andrea Buchstaller
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
Robert P. Lane
cDivision of Biology 156-29, California Institute of Technology, Pasadena, California 91125
Daniel M. Suter
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
William J. Dreyer
cDivision of Biology 156-29, California Institute of Technology, Pasadena, California 91125
Peter Sonderegger
aInstitute of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
Abbreviations used in this paper: CAM, cell adhesion molecule; Fab, antigen binding fragment; FnIII, fibronectin type III; NgCAM, neuron–glia cell adhesion molecule; NrCAM, NgCAM-related cell adhesion molecule.
Received:
December 20 1999
Revision Requested:
March 22 2000
Accepted:
April 07 2000
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Cell Biol (2000) 149 (4): 951–968.
Article history
Received:
December 20 1999
Revision Requested:
March 22 2000
Accepted:
April 07 2000
Citation
Dora Fitzli, Esther T. Stoeckli, Stefan Kunz, Kingsley Siribour, Christoph Rader, Beat Kunz, Serguei V. Kozlov, Andrea Buchstaller, Robert P. Lane, Daniel M. Suter, William J. Dreyer, Peter Sonderegger; A Direct Interaction of Axonin-1 with Ngcam-Related Cell Adhesion Molecule (Nrcam) Results in Guidance, but Not Growth of Commissural Axons. J Cell Biol 15 May 2000; 149 (4): 951–968. doi: https://doi.org/10.1083/jcb.149.4.951
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