Expression of the canine 180-kD ribosome receptor (p180) in yeast cells resulted in a marked proliferation of intracellular membranes. The type of membranes observed varied with the expression of specific portions of p180. Rough membranes predominated when the ribosome binding domain of p180 was present, whereas expression constructs lacking this region resulted in smooth membranes. Northern analysis indicated that expression of the NH2-terminal 767 amino acids (ΔCT), which include the ribosome binding domain, upregulated the transcription and translation of genes involved in exocytosis. The membranes that were proliferated were functional as these cells overcame a temperature-sensitive translocation defect. Most significantly, cells that overexpressed ΔCT and proliferated rough endoplasmic reticulum exhibited severalfold higher levels of secretion of an ectopically expressed secretory protein. We conclude that p180 expression triggers a cascade of events leading to an increase in secretory potential akin to the terminal differentiation of mammalian secretory cells and tissues.
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26 July 1999
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July 26 1999
Expression of the 180-kD Ribosome Receptor Induces Membrane Proliferation and Increased Secretory Activity in Yeast
Frank Becker,
*Department of Biological Chemistry and the Molecular Biology Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90024 and
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Laura Block-Alper,
Laura Block-Alper
*Department of Biological Chemistry and the Molecular Biology Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90024 and
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Gerald Nakamura,
Gerald Nakamura
*Department of Biological Chemistry and the Molecular Biology Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90024 and
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Josephine Harada,
Josephine Harada
*Department of Biological Chemistry and the Molecular Biology Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90024 and
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K. Dane Wittrup,
K. Dane Wittrup
‡Department of Chemical Engineering, University of Illinois, Urbana, Illinois 61801
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David I. Meyer
*Department of Biological Chemistry and the Molecular Biology Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90024 and
Address correspondence to David Meyer, Department of Biological Chemistry, University of California, Los Angeles School of Medicine, Los Angeles, CA 90024-1737. Tel.: (310) 206-3122. Fax: (310) 206-5197. E-mail: [email protected]
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Frank Becker
*Department of Biological Chemistry and the Molecular Biology Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90024 and
Laura Block-Alper
*Department of Biological Chemistry and the Molecular Biology Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90024 and
Gerald Nakamura
*Department of Biological Chemistry and the Molecular Biology Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90024 and
Josephine Harada
*Department of Biological Chemistry and the Molecular Biology Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90024 and
K. Dane Wittrup
‡Department of Chemical Engineering, University of Illinois, Urbana, Illinois 61801
Frank Becker’s present address is Genome Pharmaceutical Corporation AG, Lochhamerstrasse 29, D-82152 Martinsreid, Germany.
Address correspondence to David Meyer, Department of Biological Chemistry, University of California, Los Angeles School of Medicine, Los Angeles, CA 90024-1737. Tel.: (310) 206-3122. Fax: (310) 206-5197. E-mail: [email protected]
Frank Becker was supported by a fellowship from the Deutsche Forschungsgemeinschaft (DFG). This work was supported by a grant from National Institutes of Health (GM 38538).
1.
Abbreviations used in this paper: BPTI, bovine pancreatic trypsin inhibitor; UPR, unfolded protein response.
Received:
December 03 1998
Revision Received:
June 15 1999
Accepted:
June 17 1999
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 1999 The Rockefeller University Press
1999
J Cell Biol (1999) 146 (2): 273–284.
Article history
Received:
December 03 1998
Revision Received:
June 15 1999
Accepted:
June 17 1999
Citation
Frank Becker, Laura Block-Alper, Gerald Nakamura, Josephine Harada, K. Dane Wittrup, David I. Meyer; Expression of the 180-kD Ribosome Receptor Induces Membrane Proliferation and Increased Secretory Activity in Yeast. J Cell Biol 26 July 1999; 146 (2): 273–284. doi: https://doi.org/10.1083/jcb.146.2.273
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