Hydrolysis of inositol phospholipids by receptor stimulation activates two separate signaling pathways, one leading to the activation of protein kinase C (C kinase) via formation of diacylglycerol. The other is the inositol trisphosphate (IP3)/Ca2+ pathway and a major downstream kinase which is activated is Ca2+/calmodulin-dependent protein kinase II (CaM kinase II). To examine signaling pathways of C kinase and CaM kinase II to the cytoskeletal protein vimentin, we prepared monoclonal antibodies YT33 and MO82 which recognize the phosphorylation state of vimentin by C kinase and by CaM kinase II, respectively. Ectopic expression of constitutively active C kinase or CaM kinase II in primary cultured astrocytes by microinjection of the corresponding expression vectors induced phosphorylation of vimentin at each specific phosphorylation site, followed by reorganization of vimentin filament networks. In contrast, simultaneous activation of C kinase and CaM kinase II by inositol phospholipid hydrolysis with receptor stimulation led to an exclusive phosphorylation of vimentin at the CaM kinase II site, not at the site of C kinase. These results indicate that the intracellular targeting of C kinase and CaM kinase II signalings to vimentin is regulated separately, under physiological conditions.
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November 15 1995
Differential targeting of protein kinase C and CaM kinase II signalings to vimentin.
M Ogawara,
M Ogawara
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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N Inagaki,
N Inagaki
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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K Tsujimura,
K Tsujimura
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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Y Takai,
Y Takai
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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M Sekimata,
M Sekimata
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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M H Ha,
M H Ha
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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S Imajoh-Ohmi,
S Imajoh-Ohmi
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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S Hirai,
S Hirai
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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S Ohno,
S Ohno
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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H Sugiura
H Sugiura
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
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M Ogawara
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
N Inagaki
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
K Tsujimura
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Y Takai
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
M Sekimata
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
M H Ha
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
S Imajoh-Ohmi
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
S Hirai
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
S Ohno
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
H Sugiura
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1995) 131 (4): 1055–1066.
Citation
M Ogawara, N Inagaki, K Tsujimura, Y Takai, M Sekimata, M H Ha, S Imajoh-Ohmi, S Hirai, S Ohno, H Sugiura; Differential targeting of protein kinase C and CaM kinase II signalings to vimentin.. J Cell Biol 15 November 1995; 131 (4): 1055–1066. doi: https://doi.org/10.1083/jcb.131.4.1055
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