THE SPECIFIC VOLUME OF DRY PROTEIN

Brill , A. S.; Olin , J. S.; Siegel , B. M.

doi:10.1083/jcb.13.2.249

A. S. Brill,

A. S. Brill

From the Laboratory of Electron Microscopy, Cornell University, Ithaca, New York.

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J. S. Olin,

J. S. Olin

From the Laboratory of Electron Microscopy, Cornell University, Ithaca, New York.

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B. M. Siegel

From the Laboratory of Electron Microscopy, Cornell University, Ithaca, New York.

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A. S. Brill

From the Laboratory of Electron Microscopy, Cornell University, Ithaca, New York.

J. S. Olin

From the Laboratory of Electron Microscopy, Cornell University, Ithaca, New York.

B. M. Siegel

From the Laboratory of Electron Microscopy, Cornell University, Ithaca, New York.

Dr. Brill's present address is Biophysics Department, Yale University, New Haven. Mrs. Olin's present address is Department of Chemistry, New York University, New York

Received: December 24 1961

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1962

The specific volume of dry protein is of interest in the interpretation of electron micrographs of protein molecules. A pycnometer with helium gas as the fluid is described and measurements on dry powders of lysozyme, ovalbumin, bovine serum albumin, and fibrinogen are reported. The values obtained exceed those from determinations on solutions by an average of 1.4 per cent when the latter measurements are uncorrected for electrostriction.

1962

THE SPECIFIC VOLUME OF DRY PROTEIN

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