An ascites tumor, Sarcoma I, was transplanted to isologous and homologous mice which had been labeled with tritiated thymidine from 1 to 24 hours previously. Radioautographic preparations revealed labeled host lymphocytes emerging to mingle with the transplanted tumor and the subsequent appearance of nuclear radioactivity in the sarcoma. Sarcoma cells cultured subcutaneously or in Millipore diffusion chambers in previously labeled mice did not demonstrate significant radioactivity. Transplantation of washed, H3-thymidine-labeled lymphocytes to non-radioactive, sarcoma-bearing mice was followed by the gradual appearance of nuclear radioactivity in the sarcoma. The label in the sarcoma was entirely removed by deoxyribonuclease but not by ribonuclease treatment prior to radioautography. Intraperitoneal injections of purified, H3-thymidine-labeled sarcoma or lymphoid DNA in normal or tumor-bearing mice were followed by radioactivity appearing in sarcoma or normal peritoneal mononuclear cells. It was concluded that reutilization of DNA and its metabolites may occur in vivo, and the conditions under which reutilization may be detected are discussed.
Article|
May 01 1962
THE IN VIVO REUTILIZATION OF LYMPHOCYTIC AND SARCOMA DNA BY CELLS GROWING IN THE PERITONEAL CAVITY
William O. Rieke
William O. Rieke
From the Department of Anatomy, University of Washington School of Medicine, Seattle
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William O. Rieke
From the Department of Anatomy, University of Washington School of Medicine, Seattle
Received:
October 16 1961
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1962 by The Rockefeller Institute Press
1962
J Cell Biol (1962) 13 (2): 205–216.
Article history
Received:
October 16 1961
Citation
William O. Rieke; THE IN VIVO REUTILIZATION OF LYMPHOCYTIC AND SARCOMA DNA BY CELLS GROWING IN THE PERITONEAL CAVITY . J Cell Biol 1 May 1962; 13 (2): 205–216. doi: https://doi.org/10.1083/jcb.13.2.205
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