A COOH-terminal double lysine motif maintains type I transmembrane proteins in the ER. Proteins tagged with this motif, eg., CD8/E19 and CD4/E19, rapidly receive post-translational modifications characteristic of the intermediate compartment and partially colocalized to this organelle. These proteins also received modifications characteristic of the Golgi but much more slowly. Lectin staining localized these Golgi modified proteins to ER indicating that this motif is a retrieval signal. Differences in the subcellular distribution and rate of post-translational modification of CD8 maintained in the ER by sequences derived from a variety of ER resident proteins suggested that the efficiency of retrieval was dependent on the sequence context of the double lysine motif and that retrieval may be initiated from multiple positions along the exocytotic pathway.
Article|
April 15 1993
Retrieval of transmembrane proteins to the endoplasmic reticulum.
M R Jackson
,
M R Jackson
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
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T Nilsson
,
T Nilsson
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
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P A Peterson
P A Peterson
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
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M R Jackson
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
T Nilsson
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
P A Peterson
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1993) 121 (2): 317–333.
Citation
M R Jackson, T Nilsson, P A Peterson; Retrieval of transmembrane proteins to the endoplasmic reticulum.. J Cell Biol 15 April 1993; 121 (2): 317–333. doi: https://doi.org/10.1083/jcb.121.2.317
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