We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha-actinin. This specific isoform of alpha-actinin exhibits a more restricted pattern of expression in skeletal muscle than fast-twitch-specific isoforms of both myosin and Ca2+-ATPase. Our results provide evidence that dystrophin and myofibrillar alpha-actinin are related proteins, reinforcing the previous data concerning the sequence homologies noted between nonmuscle cytoskeletal alpha-actinin and dystrophin. In addition, we describe the first antisera directed against a specific myofibrillar skeletal muscle isoform of alpha-actinin.
Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin.
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E P Hoffman, S C Watkins, H S Slayter, L M Kunkel; Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin.. J Cell Biol 1 February 1989; 108 (2): 503–510. doi: https://doi.org/10.1083/jcb.108.2.503
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