Phospholamban, the putative regulator of the Ca2+-ATPase in cardiac sarcoplasmic reticulum, was immunolocalized in canine visceral and vascular smooth muscle. Gently disrupted tissues were labeled with an affinity-purified phospholamban polyclonal antibody and indirect immunogold, using preembedding techniques. The sarcoplasmic reticulum of smooth muscle cells was specifically labeled with patches of immunogold distributed in a nonuniform fashion, while the sarcolemma did not appear to contain any phospholamban. The outer nuclear envelopes were also observed to be heavily labeled with the affinity-purified phospholamban polyclonal antibody. These findings suggest that phospholamban may play a role in the regulation of cytoplasmic and intranuclear calcium levels in smooth muscle cells.
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August 01 1988
Localization of phospholamban in smooth muscle using immunogold electron microscopy.
D G Ferguson
,
D G Ferguson
Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, Ohio 45267-0576.
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E F Young
,
E F Young
Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, Ohio 45267-0576.
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L Raeymaekers
,
L Raeymaekers
Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, Ohio 45267-0576.
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E G Kranias
E G Kranias
Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, Ohio 45267-0576.
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D G Ferguson
Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, Ohio 45267-0576.
E F Young
Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, Ohio 45267-0576.
L Raeymaekers
Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, Ohio 45267-0576.
E G Kranias
Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, Ohio 45267-0576.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1988) 107 (2): 555–562.
Citation
D G Ferguson, E F Young, L Raeymaekers, E G Kranias; Localization of phospholamban in smooth muscle using immunogold electron microscopy.. J Cell Biol 1 August 1988; 107 (2): 555–562. doi: https://doi.org/10.1083/jcb.107.2.555
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