The glycolytic enzymes of Trypanosomatids are compartmentalized within peroxisome-like microbodies called glycosomes. Fructose bisphosphate aldolase is synthesized on free polysomes and imported into glycosomes within 5 min. Peptide mapping reveals no primary structural differences between the in vivo-synthesized protein and that made in vitro from a synthetic template. However, native aldolase from glycosomes is partially protease resistant, whereas the in vitro translation product is not. Pulse-chase results indicate that aldolase in bloodstream trypanosomes has a much longer half-life than in the procyclic tsetse fly form.
Article| December 01 1987
Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei.
C E Clayton
Rockefeller University, New York 10021-6399.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1987) 105 (6): 2649–2654.
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C E Clayton; Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei.. J Cell Biol 1 December 1987; 105 (6): 2649–2654. doi: https://doi.org/10.1083/jcb.105.6.2649
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