Fluorescence redistribution after photobleaching (FRAP) was used to examine the role of actin and myosin in the transport of dextrans through the nuclear pore complex. Anti-actin antibodies added to isolated rat liver nuclei significantly reduced the flux rate of fluorescently labeled 64-kD dextrans. The addition of 3 mM ATP to nuclei, which enhances the flux rate in control nuclei by approximately 250%, had no enhancement effect in the presence of either anti-actin or anti-myosin antibody. Phalloidin (10 microM) and cytochalasin D (1 micrograms/ml) individually inhibited the ATP stimulation of transport. Rabbit serum, anti-fibronectin, and anti-lamins A and C antibodies had no effect on transport. These results suggest a model for nuclear transport in which actin/myosin are involved in an ATP-dependent process that alters the effective transport rate across the nuclear pore complex.
Article| March 01 1986
Nuclear actin and myosin as control elements in nucleocytoplasmic transport.
L W Jiang
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1986) 102 (3): 859–862.
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M Schindler, L W Jiang; Nuclear actin and myosin as control elements in nucleocytoplasmic transport.. J Cell Biol 1 March 1986; 102 (3): 859–862. doi: https://doi.org/10.1083/jcb.102.3.859
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