In the preceding article we described a polyclonal antibody that recognizes cSc-1, a major polypeptide component of the chicken mitotic chromosome scaffold. This polypeptide was shown to be chicken topoisomerase II. In the experiments described in the present article we use indirect immunofluorescence and immunoelectron microscopy to examine the distribution of topoisomerase II within intact chromosomes. We also describe a simple experimental protocol that differentiates antigens that are interspersed along the chromatin fiber from those that occupy restricted domains within the chromosome. These experiments indicate that the distribution of the enzyme appears to be independent of the bulk chromatin. Our data suggest that topoisomerase II is bound to the bases of the radial loop domains of mitotic chromosomes.
Article| May 01 1985
Localization of topoisomerase II in mitotic chromosomes.
In Special Collection: JCB65: Nuclear and Chromatin Biology
W C Earnshaw
M M Heck
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1985) 100 (5): 1716–1725.
W C Earnshaw, M M Heck; Localization of topoisomerase II in mitotic chromosomes.. J Cell Biol 1 May 1985; 100 (5): 1716–1725. doi: https://doi.org/10.1083/jcb.100.5.1716
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