The eggs of most or all animals are thought to be activated after fertilization by a transient increase in free cytosolic Ca2+ concentration ([Ca2+]i). We have applied Ca2+-selective microelectrodes to detect such an increase in fertilized eggs of the frog, Xenopus laevis. As observed with an electrode in the animal hemisphere, [Ca2+]i increased from 0.4 to 1.2 microM over the course of 2 min after fertilization, and returned to its original value during the next 10 min. No further changes in [Ca2+]i were detected through the first cleavage division. In eggs impaled with two Ca2+ electrodes, the Ca2+ pulse was observed to travel as a wave from the animal to the vegetal hemisphere, propagating at a rate of approximately 10 microns/s across the animal hemisphere. The apparent delay between the start of the fertilization potential and initiation of the Ca2+ wave at the sperm entry site as approximately 1 min. Through these observations describe only the behavior of subcortical [Ca2+]i, we suggest that our data represent the subcortical extension of the cortical Ca2+ wave thought to trigger cortical granule exocytosis, and we present evidence that both the timing and magnitude of the Ca2+ pulse we observed are consistent with this identity. This first quantification of subcortical [Ca2+]i during fertilization indicates that the Ca2+ transient is available to regulate processes (e.g., protein synthesis) in the subcortical cytosol.
Article| April 01 1985
An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.
W B Busa
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1985) 100 (4): 1325–1329.
W B Busa, R Nuccitelli; An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.. J Cell Biol 1 April 1985; 100 (4): 1325–1329. doi: https://doi.org/10.1083/jcb.100.4.1325
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