Murphy and Montell. Vol. 133, No. 3, May 1996. Pages 617–630.

A corrected Table II and legend to Fig. 5 appear below.

Table II

Lethality/Viability of Gal4 Constructs with UAS-Rac, Cdc42, or RhoL

UAS construct458306198Y212hs-GAL4
RhoLV20  Lethal  Lethal  Lethal  Viable  Lethal 
RhoLN25  Viable  Viable  Viable  Viable  Viable 
RacV12  Lethal  Lethal  Lethal  Lethal  Lethal 
RacN17  Viable  Viable  Viable  Viable  Viable 
Cdc42V12  Viable  Lethal  Lethal  Lethal  Semi-lethal 
Cdc42N17  Viable  Viable  Lethal  Viable  Viable 
Embyronic expression  None  Salivary gland  Salivary gland  Not tested  Not tested 
Larval expression  Moderate, general  Strong, general  Strong, general  Not tested  Not tested 
UAS construct458306198Y212hs-GAL4
RhoLV20  Lethal  Lethal  Lethal  Viable  Lethal 
RhoLN25  Viable  Viable  Viable  Viable  Viable 
RacV12  Lethal  Lethal  Lethal  Lethal  Lethal 
RacN17  Viable  Viable  Viable  Viable  Viable 
Cdc42V12  Viable  Lethal  Lethal  Lethal  Semi-lethal 
Cdc42N17  Viable  Viable  Lethal  Viable  Viable 
Embyronic expression  None  Salivary gland  Salivary gland  Not tested  Not tested 
Larval expression  Moderate, general  Strong, general  Strong, general  Not tested  Not tested 

 

Figure 5. Changes in F-actin distribution during normal border cell migration and effects of altered Rho proteins on border cells. (A and K) Wild-type stage 10 egg chambers stained for F-actin. (B) Nomarski optics image of slbo1/CyO stage 10 egg chamber stained for β-galactosidase activity. (C) High power confocal micrographs of wild-type border cells initiating migration in stage 9 (C) and after migration in stage 10 (D), stained for F-actin. The triangle indicates one filopodium in C. C and D are high power images of the same egg chambers shown in Fig. 1, H and I. (E) F-actin staining of a representative egg chamber expressing RacN17 in border cells and posterior follicle cells (Gal4-306;UAS-RacN17). (F) β-Galactosidase activity staining of a stage 10 egg chamber (Gal4-306;UAS-RacN17; slbo1/+), showing that the cells at the anterior tip are border cells. (G) High power confocal micrograph of stage 10 border cells from Gal4-306;UAS-RacN17 stained for F-actin. Note the lack of cellular extensions of filopodia. (H) Nuclear staining of G. (I) F-actin staining of a stage 9 egg chamber expressing dominant-negative RhoL. (J) β-Galactosidase activity staining in a similar egg chamber. Arrows indicate border cells.