Light and electron microscope immunohistochemical methods were used to study the distribution of several proteins in rat skeletal muscle. The aims were to identify components of muscle fiber basement membrane and to compare the small fraction (0.1%) of the basement membrane that extends through the synaptic cleft at the neuromuscular junction with the remaining, extrasynaptic portion. Synaptic basement membrane is functionally specialized and plays important roles in neuromuscular function and regeneration. Laminin, fibronectin, collagen IV, collagen V, and a collagenous protein (high-salt-soluble protein [HSP]) are all present in muscle fiber basement membrane. Laminin and collagen IV are concentrated in basal lamina (the feltlike, inner layer of the basement membrane) and are shared by synaptic and extrasynaptic regions. Fibronectin, also present synaptically and extrasynaptically, is present in basal lamina and in the overlying reticular lamina. Collagen V and HSP are present throughout extrasynaptic basement membrane but are absent from synaptic sites; HSP is concentrated in the reticular lamina and on the outer surface of the basal lamina. These results, together with experiments reported previously (Sanes and Hall, 1979. J. Cell Biol: 83:357--370), provide examples of three classes of components in muscle fiber basement membrane--synaptic, extrasynaptic, and shared.
Skip Nav Destination
Article navigation
1 May 1982
Article|
May 01 1982
Laminin, fibronectin, and collagen in synaptic and extrasynaptic portions of muscle fiber basement membrane.
J R Sanes
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1982) 93 (2): 442–451.
Citation
J R Sanes; Laminin, fibronectin, and collagen in synaptic and extrasynaptic portions of muscle fiber basement membrane.. J Cell Biol 1 May 1982; 93 (2): 442–451. doi: https://doi.org/10.1083/jcb.93.2.442
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
Advertisement