Improvement in the Histochemical Localization of Leucine Aminopeptidase with a New Substrate, L-Leucyl-4-Methoxy-2-Naphthylamide

Nachlas , Marvin M.; Monis , Benito; Rosenblatt , David; Seligman , Arnold M.

doi:10.1083/jcb.7.2.261

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Article| April 01 1960

Improvement in the Histochemical Localization of Leucine Aminopeptidase with a New Substrate, L-Leucyl-4-Methoxy-2-Naphthylamide

Marvin M. Nachlas,

Marvin M. Nachlas

From the Department of Surgery, Sinai Hospital of Baltimore, Inc., and the Departments of Surgery and Chemistry, The Johns Hopkins University, Baltimore

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Benito Monis,

Benito Monis

From the Department of Surgery, Sinai Hospital of Baltimore, Inc., and the Departments of Surgery and Chemistry, The Johns Hopkins University, Baltimore

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David Rosenblatt,

David Rosenblatt

From the Department of Surgery, Sinai Hospital of Baltimore, Inc., and the Departments of Surgery and Chemistry, The Johns Hopkins University, Baltimore

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Arnold M. Seligman

From the Department of Surgery, Sinai Hospital of Baltimore, Inc., and the Departments of Surgery and Chemistry, The Johns Hopkins University, Baltimore

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Author and Article Information

Marvin M. Nachlas

From the Department of Surgery, Sinai Hospital of Baltimore, Inc., and the Departments of Surgery and Chemistry, The Johns Hopkins University, Baltimore

Benito Monis

From the Department of Surgery, Sinai Hospital of Baltimore, Inc., and the Departments of Surgery and Chemistry, The Johns Hopkins University, Baltimore

David Rosenblatt

From the Department of Surgery, Sinai Hospital of Baltimore, Inc., and the Departments of Surgery and Chemistry, The Johns Hopkins University, Baltimore

Arnold M. Seligman

From the Department of Surgery, Sinai Hospital of Baltimore, Inc., and the Departments of Surgery and Chemistry, The Johns Hopkins University, Baltimore

Received: October 18 1959

Copyright, 1960, by The Rockefeller Institute Press

1960

J Biophys and Biochem Cytol (1960) 7 (2): 261–264.

https://doi.org/10.1083/jcb.7.2.261

A new method for the histochemical demonstration of leucine aminopeptidase in fresh frozen sections was developed with the substrate L-leucyl-4-methoxy-2-naphthylamide. The superior enzyme localization is due to the more rapid rate of coupling of the hydrolysis product, 4-methoxy-2-naphthylamine as compared to 2-naphthylamine itself, and to the low lipid solubility and high substantivity for protein of the copper chelate of the dye formed on coupling with tetrazotized diorthoanisidine. A comparison of the old and the new method is illustrated, and a description is given of the localization of leucine aminopeptidase in the tissues of the rat and man.

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Copyright, 1960, by The Rockefeller Institute Press

1960

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Improvement in the Histochemical Localization of Leucine Aminopeptidase with a New Substrate, L-Leucyl-4-Methoxy-2-Naphthylamide

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