Microtubules isolated from brain extracts by in vitro assembly (1, 19, 23) are composed principally of two tubulins and two high molecular weight proteins (microtubule-associated proteins [MAPS] 1 and 2) (2,5,7,20). Recently, it was demonstrated that in vitro-assembled brain microtubules (neurotubules) are coated with filaments (5, 7) which are similar to the filaments attached to neurotubules in situ (4, 15, 21, 24, 25), and it was suggested that the filaments are composed of the higher molecular weight MAPs (5, 7, 12). In this study, microtubules were assembled in the presence and absence of the MAPs, and thin sections of the microtubules were examined by electron microscopy. The results show that the filaments only occur on microtubules assembled in the presence of the MAPs and it is therefore concluded that the filaments are composed of the high molecular weight MAP's.
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1 April 1975
Article|
April 01 1975
Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules
WL Dentler
S Granett
JL Rosenbaum
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1975
J Cell Biol (1975) 65 (1): 237–241.
Citation
WL Dentler, S Granett, JL Rosenbaum; Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules . J Cell Biol 1 April 1975; 65 (1): 237–241. doi: https://doi.org/10.1083/jcb.65.1.237
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