In this report we describe two nonallelic Mendelian protoporphyrin accumulating mutants brs-1 and brc-1. Results of experiments with these mutants lead us to postulate that porphyrin biosynthesis branches into light and dark steps between protoporphyrin-IX and magnesium protoporphyrin. We hypothesize that the brc locus controls a dark step while the brs locus either controls a step in the main pathway before the branch or mediates the preparation of the magnesium ion for its insertion into protoporphyrin-IX. The brs-1 mutant is thought to be light sensitive because a block prior to the branch point in the porphyrin pathway prevents chlorophyll formation in either the light or the dark. The brc-1 mutant, which also accumulates protoporphyrin in the dark, forms chlorophyll and chloroplast lamellae when transferred to the light, showing that function of the porphyrin pathway is normal in the light.
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1 December 1974
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December 01 1974
GENETIC CONTROL OF CHLOROPHYLL BIOSYNTHESIS IN CHLAMYDOMONAS : Analysis of Mutants at Two Loci Mediating the Conversion of Protoporphyrin-IX to Magnesium Protoporphyrin
Wei-Yeh Wang,
Wei-Yeh Wang
From the Departments of Botany and Zoology, Duke University, Durham, North Carolina 27706
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Wenan Lee Wang,
Wenan Lee Wang
From the Departments of Botany and Zoology, Duke University, Durham, North Carolina 27706
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John E. Boynton,
John E. Boynton
From the Departments of Botany and Zoology, Duke University, Durham, North Carolina 27706
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Nicholas W. Gillham
Nicholas W. Gillham
From the Departments of Botany and Zoology, Duke University, Durham, North Carolina 27706
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Wei-Yeh Wang
From the Departments of Botany and Zoology, Duke University, Durham, North Carolina 27706
Wenan Lee Wang
From the Departments of Botany and Zoology, Duke University, Durham, North Carolina 27706
John E. Boynton
From the Departments of Botany and Zoology, Duke University, Durham, North Carolina 27706
Nicholas W. Gillham
From the Departments of Botany and Zoology, Duke University, Durham, North Carolina 27706
Received:
March 04 1974
Revision Received:
July 22 1974
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1974 by The Rockefeller University Press
1974
J Cell Biol (1974) 63 (3): 806–823.
Article history
Received:
March 04 1974
Revision Received:
July 22 1974
Citation
Wei-Yeh Wang, Wenan Lee Wang, John E. Boynton, Nicholas W. Gillham; GENETIC CONTROL OF CHLOROPHYLL BIOSYNTHESIS IN CHLAMYDOMONAS : Analysis of Mutants at Two Loci Mediating the Conversion of Protoporphyrin-IX to Magnesium Protoporphyrin . J Cell Biol 1 December 1974; 63 (3): 806–823. doi: https://doi.org/10.1083/jcb.63.3.806
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