A DEMONSTRATION OF SEVERAL DEOXYRIBONUCLEASE ACTIVITIES IN MAMMALIAN CELL MITOCHONDRIA

Durphy , Michael; Manley , Paul N.; Friedberg , Errol C.

doi:10.1083/jcb.62.3.695

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Article| September 01 1974

A DEMONSTRATION OF SEVERAL DEOXYRIBONUCLEASE ACTIVITIES IN MAMMALIAN CELL MITOCHONDRIA

Michael Durphy,

Michael Durphy

From the Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305

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Paul N. Manley,

Paul N. Manley

From the Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305

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Errol C. Friedberg

From the Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305

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Author and Article Information

Michael Durphy

From the Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305

Paul N. Manley

From the Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305

Errol C. Friedberg

From the Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305

Received: September 04 1973

Revision Received: March 18 1974

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1974

J Cell Biol (1974) 62 (3): 695–706.

https://doi.org/10.1083/jcb.62.3.695

Extracts of purified mitochondria from adult rabbit liver and kidney have been prepared by lysis with Triton X-100. Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di- and triphosphates, and SH-group inhibitors reveal the existence of at least five distinguishable deoxyribonuclease activities in these extracts.

Assay of lysosomal and mitochondrial enzyme markers indicates no significant lysosomal contamination of the mitochondrial extracts. Further studies also suggest that the alkaline deoxyribonuclease activity is specifically located in or in association with mitochondria.

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A DEMONSTRATION OF SEVERAL DEOXYRIBONUCLEASE ACTIVITIES IN MAMMALIAN CELL MITOCHONDRIA

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