The ultrastructure of isolated cell walls of Saccharomyces cerevisiae from the log and stationary phases of growth was studied after treatment with the following enzymes: purified endo-ß-(1 → 3)-glucanase and endo-ß-(1 → 6)-glucanase produced by Bacillus circulans; purified exo-ß-glucanase and endo-ß-(1 → 3)-glucanase produced by Schizosaccharomyces versatilis; commercial Pronase. While exo-ß-glucanase from S. versatilis had no electron microscopically detectable effect on the walls, Pronase removed part of the external amorphous wall material disclosing an amorphous wall layer in which fibrils were indistinctly visible. Amorphous wall material was completely removed by the effect of either endo-ß-(1 → 3)- or endo-ß-(1 → 6)-glucanase of B. circulans or by a mixture of the two enzymes. As a result of these treatments a continuous fibrillar component appeared, composed of densely interwoven microfibrils resisting further action by both of the B. circulans enzymes. The fibrillar wall component was also demonstrated in untreated cell walls by electron microscopy after negative staining. Because of the complete disappearance of the fibrils following treatment with the S. versatilis endo-ß-(1 → 3)-glucanase it can be concluded that this fibrillar component is composed of ß-(1 → 3)-linked glucan. Bud scars were the only wall structures resistant to the effect of the latter enzyme.
Skip Nav Destination
Article navigation
1 July 1974
Article|
July 01 1974
DEMONSTRATION OF A FIBRILLAR COMPONENT IN THE CELL WALL OF THE YEAST SACCHAROMYCES CEREVISIAE AND ITS CHEMICAL NATURE
Marie Kopecká,
Marie Kopecká
From the Department of General Biology, Faculty of Medicine, J. E. Purkyné University, Brno, Czechoslovakia, and the Department of Food Science and Technology, University of California, Davis, California 95616
Search for other works by this author on:
H. J. Phaff,
H. J. Phaff
From the Department of General Biology, Faculty of Medicine, J. E. Purkyné University, Brno, Czechoslovakia, and the Department of Food Science and Technology, University of California, Davis, California 95616
Search for other works by this author on:
G. H. Fleet
G. H. Fleet
From the Department of General Biology, Faculty of Medicine, J. E. Purkyné University, Brno, Czechoslovakia, and the Department of Food Science and Technology, University of California, Davis, California 95616
Search for other works by this author on:
Marie Kopecká
From the Department of General Biology, Faculty of Medicine, J. E. Purkyné University, Brno, Czechoslovakia, and the Department of Food Science and Technology, University of California, Davis, California 95616
H. J. Phaff
From the Department of General Biology, Faculty of Medicine, J. E. Purkyné University, Brno, Czechoslovakia, and the Department of Food Science and Technology, University of California, Davis, California 95616
G. H. Fleet
From the Department of General Biology, Faculty of Medicine, J. E. Purkyné University, Brno, Czechoslovakia, and the Department of Food Science and Technology, University of California, Davis, California 95616
Received:
November 12 1973
Revision Received:
March 06 1974
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1974 by The Rockefeller University Press
1974
J Cell Biol (1974) 62 (1): 66–76.
Article history
Received:
November 12 1973
Revision Received:
March 06 1974
Citation
Marie Kopecká, H. J. Phaff, G. H. Fleet; DEMONSTRATION OF A FIBRILLAR COMPONENT IN THE CELL WALL OF THE YEAST SACCHAROMYCES CEREVISIAE AND ITS CHEMICAL NATURE . J Cell Biol 1 July 1974; 62 (1): 66–76. doi: https://doi.org/10.1083/jcb.62.1.66
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement