Early events in the biosynthesis of liver catalase were studied on female rats receiving [3H]leucine or [3H]δ-aminolevulinic acid or a mixture of [3H]leucine with [14C]δ-aminolevulinic acid by intraportal injection. Catalase antigen was selectively separated from homogenates by immunoprecipitation, both without and after partial purification of the enzyme. Label from both precursors appeared first in immunoprecipitable material which was lost upon purification of catalase; the label subsequently became associated with material indistinguishable from catalase. Kinetic analysis of the results indicates that the nonpurifiable material identified by early labeling consists of two distinct biosynthetic intermediates, the first lacking heme and representing about 1.6% of the total catalase content or 13 µg/g liver, the second containing heme and representing about 0.5% of the total catalase content or 4 µg/g liver. The first intermediate migrates at the same rate as catalase upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and therefore has a monomeric molecular weight of about 60,000.
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1 November 1973
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November 01 1973
THE SYNTHESIS AND TURNOVER OF RAT LIVER PEROXISOMES : IV. Biochemical Pathway of Catalase Synthesis
Paul B. Lazarow,
Paul B. Lazarow
From The Rockefeller University, New York 10021.
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Christian de Duve
Christian de Duve
From The Rockefeller University, New York 10021.
Search for other works by this author on:
Paul B. Lazarow
From The Rockefeller University, New York 10021.
Christian de Duve
From The Rockefeller University, New York 10021.
Dr. Lazarow's present address is Department of Biological Sciences, Stanford University, Stanford, California 94305.
Received:
April 17 1973
Revision Received:
June 28 1973
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1973 by The Rockefeller University Press
1973
J Cell Biol (1973) 59 (2): 491–506.
Article history
Received:
April 17 1973
Revision Received:
June 28 1973
Citation
Paul B. Lazarow, Christian de Duve; THE SYNTHESIS AND TURNOVER OF RAT LIVER PEROXISOMES : IV. Biochemical Pathway of Catalase Synthesis . J Cell Biol 1 November 1973; 59 (2): 491–506. doi: https://doi.org/10.1083/jcb.59.2.491
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