The three Golgi fractions isolated from rat liver homogenates by the procedure given in the companion paper account for 6–7% of the protein of the total microsomal fraction used as starting preparation. The lightest, most homogeneous Golgi fraction (GF1) lacks typical "microsomal" activities, e.g., glucose-6-phosphatase, NADPH-cytochrome c-reductase, and cytochrome P-450. The heaviest, most heterogeneous fraction (GF3) is contaminated by endoplasmic reticulum membranes to the extent of ∼15% of its protein. The three fractions taken together account for nearly all the UDP-galactose: N-acetyl-glucosamine galactosyltransferase of the parent microsomal fraction, and for ∼70% of the activity of the original homogenate. Omission of the ethanol treatment of the animals reduces the recovery by half. The transferase activity is associated with the membranes of the Golgi elements, not with their content. Galactose is transferred not only to N-acetyl-glucosamine but also to an unidentified lipid-soluble component.
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1 October 1973
Article|
October 01 1973
GOLGI FRACTIONS PREPARED FROM RAT LIVER HOMOGENATES : II. Biochemical Characterization
J. J. M. Bergeron,
J. J. M. Bergeron
From The Rockefeller University, New York 10021.
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J. H. Ehrenreich,
J. H. Ehrenreich
From The Rockefeller University, New York 10021.
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P. Siekevitz,
P. Siekevitz
From The Rockefeller University, New York 10021.
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G. E. Palade
G. E. Palade
From The Rockefeller University, New York 10021.
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J. J. M. Bergeron
From The Rockefeller University, New York 10021.
J. H. Ehrenreich
From The Rockefeller University, New York 10021.
P. Siekevitz
From The Rockefeller University, New York 10021.
G. E. Palade
From The Rockefeller University, New York 10021.
Dr. Bergeron's present address is the National Institute for Medical Research, Mill Hill, London N.W. 7, England. Dr. Ehrenreich's present address is the College at Old Westbury, Old Westbury, New York 11568.
Received:
February 12 1973
Revision Received:
June 07 1973
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1973 by The Rockefeller University Press
1973
J Cell Biol (1973) 59 (1): 73–88.
Article history
Received:
February 12 1973
Revision Received:
June 07 1973
Citation
J. J. M. Bergeron, J. H. Ehrenreich, P. Siekevitz, G. E. Palade; GOLGI FRACTIONS PREPARED FROM RAT LIVER HOMOGENATES : II. Biochemical Characterization . J Cell Biol 1 October 1973; 59 (1): 73–88. doi: https://doi.org/10.1083/jcb.59.1.73
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