The "circle analysis" method of Williams (1969) and a new improved method employing hypothetical grains described in the previous paper have been used to analyze the distribution of autoradiographic grains over erythroid bone marrow cells labeled with radioactive iron, 55Fe. The resolution obtainable with this isotope was determined by measuring the distribution of grains about a thin line source. This distribution was also used in calculation of the circle size for the Williams's analysis and the distances of hypothetical grains for the new method. The new method provides estimates for the amount of activity in the regions of condensed and extended nuclear chromatin and for the concentration of isotope at the junction between these two areas. The possible significance of activity in this junctional region is discussed.
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1 April 1973
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April 01 1973
ELECTRON MICROSCOPE AUTORADIOGRAPHY OF ERYTHROID CELLS USING RADIOACTIVE IRON
D. M. Parry,
D. M. Parry
From the Biophysics Division, Institute of Cancer Research, Sutton, Surrey, England
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N. M. Blackett
N. M. Blackett
From the Biophysics Division, Institute of Cancer Research, Sutton, Surrey, England
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D. M. Parry
From the Biophysics Division, Institute of Cancer Research, Sutton, Surrey, England
N. M. Blackett
From the Biophysics Division, Institute of Cancer Research, Sutton, Surrey, England
Received:
May 24 1972
Revision Received:
November 13 1972
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1973 by The Rockefeller University Press
1973
J Cell Biol (1973) 57 (1): 16–26.
Article history
Received:
May 24 1972
Revision Received:
November 13 1972
Citation
D. M. Parry, N. M. Blackett; ELECTRON MICROSCOPE AUTORADIOGRAPHY OF ERYTHROID CELLS USING RADIOACTIVE IRON . J Cell Biol 1 April 1973; 57 (1): 16–26. doi: https://doi.org/10.1083/jcb.57.1.16
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