STUDIES ON ISOLATED MEMBRANES OF AZUROPHIL AND SPECIFIC GRANULES FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES

Nachman , Ralph; Hirsch , James G.; Baggiolini , Marco

doi:10.1083/jcb.54.1.133

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Article| July 01 1972

STUDIES ON ISOLATED MEMBRANES OF AZUROPHIL AND SPECIFIC GRANULES FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES

Ralph Nachman,

Ralph Nachman

From The New York Hospital-Cornell Medical Center, New York 10021, The Rockefeller University, New York 10021, and the Research Institute, Wander Limited, CH-3001 Berne, Switzerland

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James G. Hirsch,

James G. Hirsch

From The New York Hospital-Cornell Medical Center, New York 10021, The Rockefeller University, New York 10021, and the Research Institute, Wander Limited, CH-3001 Berne, Switzerland

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Marco Baggiolini

From The New York Hospital-Cornell Medical Center, New York 10021, The Rockefeller University, New York 10021, and the Research Institute, Wander Limited, CH-3001 Berne, Switzerland

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Author and Article Information

Ralph Nachman

From The New York Hospital-Cornell Medical Center, New York 10021, The Rockefeller University, New York 10021, and the Research Institute, Wander Limited, CH-3001 Berne, Switzerland

James G. Hirsch

From The New York Hospital-Cornell Medical Center, New York 10021, The Rockefeller University, New York 10021, and the Research Institute, Wander Limited, CH-3001 Berne, Switzerland

Marco Baggiolini

From The New York Hospital-Cornell Medical Center, New York 10021, The Rockefeller University, New York 10021, and the Research Institute, Wander Limited, CH-3001 Berne, Switzerland

Received: January 25 1972

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1972

J Cell Biol (1972) 54 (1): 133–140.

https://doi.org/10.1083/jcb.54.1.133

Membranes were prepared from rabbit polymorphonuclear leukocyte azurophil and specific granules separated by zonal differential centrifugation. The two types of granule membranes were quite similar in ultrastructural appearance, but they showed distinct differences in cholesterol-phospholipid ratios and in protein components demonstrable in polyacrylamide gels.

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STUDIES ON ISOLATED MEMBRANES OF AZUROPHIL AND SPECIFIC GRANULES FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES

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