A correlation had previously been established between actomyosin content of homogenized skeletal muscle cell segments as determined by extraction in strong salt solution and the ability of those segments to empty when extracted with buffered water. In this study, we examined the ability of certain compounds to inhibit the process of emptying. Adenosine triphosphate (ATP) and adenosine diphosphate (ADP), which dissociate actomyosin, inhibited the process of emptying, while adenosine monophosphate (AMP) which does not dissociate actomyosin, did not. We conclude that the formation of actomyosin is a necessary prerequisite for emptying and not just a secondary effect. Polyvalent cations were also found to inhibit emptying. The inhibition was reversible by washing with a solution of NaCl-histidine or with chelating agents, ethylenediaminetetraacetate (EDTA) and ethylene-glycol-bis(ß-amino-ethyl ether) tetraacetic acid (EGTA). A factor(s) solubilized from aged muscle functions as an inhibitory agent; the suggestion is made that this factor(s) may be a polyvalent cation.
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1 May 1971
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May 01 1971
INHIBITION OF EMPTYING OF SKELETAL MUSCLE CELL SEGMENTS BY ADENINE NUCLEOTIDES AND POLYVALENT CATIONS
E. A. Comissiong,
E. A. Comissiong
From the Department of Food Science and Technology, University of Massachusetts, Amherst, Massachusetts 01002.
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D. W. Stanley,
D. W. Stanley
From the Department of Food Science and Technology, University of Massachusetts, Amherst, Massachusetts 01002.
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H. O. Hultin
H. O. Hultin
From the Department of Food Science and Technology, University of Massachusetts, Amherst, Massachusetts 01002.
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E. A. Comissiong
From the Department of Food Science and Technology, University of Massachusetts, Amherst, Massachusetts 01002.
D. W. Stanley
From the Department of Food Science and Technology, University of Massachusetts, Amherst, Massachusetts 01002.
H. O. Hultin
From the Department of Food Science and Technology, University of Massachusetts, Amherst, Massachusetts 01002.
Dr. Stanley's present address is the Department of Food Science, University of Guelph, Guelph, Ontario
Received:
July 28 1970
Revision Received:
October 02 1970
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1971 by The Rockefeller University Press
1971
J Cell Biol (1971) 49 (2): 430–437.
Article history
Received:
July 28 1970
Revision Received:
October 02 1970
Citation
E. A. Comissiong, D. W. Stanley, H. O. Hultin; INHIBITION OF EMPTYING OF SKELETAL MUSCLE CELL SEGMENTS BY ADENINE NUCLEOTIDES AND POLYVALENT CATIONS . J Cell Biol 1 May 1971; 49 (2): 430–437. doi: https://doi.org/10.1083/jcb.49.2.430
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