Klebsiella aerogenes was grown in chemostat culture with the pH controlled to ±0.01 and temperature to ±0.1°C. The oxygen tension of the culture was regulated by changing the partial pressure of oxygen in the gas phase and recorded by means of an oxygen electrode. Reduced pyridine nucleotide was monitored continuously in the culture by means of direct fluorimetry. On applying an anaerobic shock to the culture, damped oscillations in pyridine nucleotide fluorescence were obtained. Further anaerobic shocks decreased the damping and eventually gave rise to undamped oscillations of a 2–3 min period which continued for several days. These oscillations were paralleled by oscillations of the same frequency in respiration rate. The amplitude of the oscillations in the respiration rate was equivalent to only 1% of the total steady-state respiration, whereas that of pyridine nucleotide oscillations was equivalent to 10% of the total aerobic/anaerobic fluorescence response. The oscillations ceased on interrupting the glucose feed but restarted on adding excess glucose to the culture. Addition of succinate also restarted the oscillations so that they appear not to be of glycolytic origin. The frequency of oscillations varied with growth rate and conditions. Oscillations of much lower frequency were obtained under limited-oxygen and anaerobic conditions than under fully aerobic conditions. Under glucose-limited conditions, fluctuations were found in adenosine triphosphate (ATP) content which were in phase with the pyridine nucleotide oscillations, but under nitrogen-limited growth conditions no such fluctuations in ATP were observed. The primary oscillating pathway could not be identified but the mechanism would appear to be quite different from that involved in oscillations observed in yeast cells. The synchronization of oscillations and observations of negative damping could be explained by a syntalysis effect.
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1 June 1970
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June 01 1970
UNDAMPED OSCILLATIONS OF PYRIDINE NUCLEOTIDE AND OXYGEN TENSION IN CHEMOSTAT CULTURES OF KLEBSIELLA AEROGENES
David E. F. Harrison
David E. F. Harrison
From the Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania 19104
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David E. F. Harrison
From the Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania 19104
Received:
September 29 1969
Revision Received:
January 22 1970
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1970 by The Rockefeller University Press
1970
J Cell Biol (1970) 45 (3): 514–521.
Article history
Received:
September 29 1969
Revision Received:
January 22 1970
Citation
David E. F. Harrison; UNDAMPED OSCILLATIONS OF PYRIDINE NUCLEOTIDE AND OXYGEN TENSION IN CHEMOSTAT CULTURES OF KLEBSIELLA AEROGENES . J Cell Biol 1 June 1970; 45 (3): 514–521. doi: https://doi.org/10.1083/jcb.45.3.514
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