Healing skin wounds were studied in a series of parabiotic rats. The femurs of one parabiont of each pair were shielded whilst both animals were given 800 r from a Co60 source. The animals were wounded 3 days after irradiation. Each animal with partially shielded marrow was then given tritiated thymidine intraperitoneally daily while the cross-circulation was arrested by clamping. After the thymidine-3H had cleared the blood, the clamp was released. Animals were sacrificed, and wounds were prepared for radioautography 1, 2, and 6 days after wounding. In the wounds of the shielded animals thymidine-3H was observed in epidermis, endothelium, leukocytes, fibroblasts, and mast cells. Only neutrophilic leukocytes, monocytes, and lymphocytes were labeled, as determined by light and electron microscope radioautography, in the wounds of each nonshielded parabiont. None of the many fibroblasts present were found to contain label in the wounds of the nonshielded parabionts through the 6 day period. These observations provide further evidence that wound fibroblasts do not arise from hematogenous precursors and, therefore, must arise from adjacent connective tissue cells.
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1 March 1970
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March 01 1970
WOUND HEALING AND COLLAGEN FORMATION : VI. The Origin of the Wound Fibroblast Studied in Parabiosis
Russell Ross,
Russell Ross
From the Departments of Pathology and Biological Structure, University of Washington, Seattle, Washington 98105
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Newton B. Everett,
Newton B. Everett
From the Departments of Pathology and Biological Structure, University of Washington, Seattle, Washington 98105
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Ruth Tyler
Ruth Tyler
From the Departments of Pathology and Biological Structure, University of Washington, Seattle, Washington 98105
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Russell Ross
From the Departments of Pathology and Biological Structure, University of Washington, Seattle, Washington 98105
Newton B. Everett
From the Departments of Pathology and Biological Structure, University of Washington, Seattle, Washington 98105
Ruth Tyler
From the Departments of Pathology and Biological Structure, University of Washington, Seattle, Washington 98105
Received:
September 10 1969
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1970 by The Rockefeller University Press
1970
J Cell Biol (1970) 44 (3): 645–654.
Article history
Received:
September 10 1969
Citation
Russell Ross, Newton B. Everett, Ruth Tyler; WOUND HEALING AND COLLAGEN FORMATION : VI. The Origin of the Wound Fibroblast Studied in Parabiosis . J Cell Biol 1 March 1970; 44 (3): 645–654. doi: https://doi.org/10.1083/jcb.44.3.645
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