THE SUBCELLULAR LOCALIZATION OF CALCIUM ION IN MAMMALIAN MYOCARDIUM

Legato , Marianne J.; Langer , Glenn A.

doi:10.1083/jcb.41.2.401

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Article| May 01 1969

THE SUBCELLULAR LOCALIZATION OF CALCIUM ION IN MAMMALIAN MYOCARDIUM

Marianne J. Legato,

Marianne J. Legato

From the Departments of Medicine, Roosevelt Hospital and Columbia University, College of Physicians and Surgeons, New York, New York, and the Departments of Medicine and Physiology, the Los Angeles County Cardiovascular Research Laboratory, University of California and Los Angeles Center for the Health Sciences, Los Angeles, California

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Glenn A. Langer

From the Departments of Medicine, Roosevelt Hospital and Columbia University, College of Physicians and Surgeons, New York, New York, and the Departments of Medicine and Physiology, the Los Angeles County Cardiovascular Research Laboratory, University of California and Los Angeles Center for the Health Sciences, Los Angeles, California

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Author and Article Information

Marianne J. Legato

From the Departments of Medicine, Roosevelt Hospital and Columbia University, College of Physicians and Surgeons, New York, New York, and the Departments of Medicine and Physiology, the Los Angeles County Cardiovascular Research Laboratory, University of California and Los Angeles Center for the Health Sciences, Los Angeles, California

Glenn A. Langer

From the Departments of Medicine, Roosevelt Hospital and Columbia University, College of Physicians and Surgeons, New York, New York, and the Departments of Medicine and Physiology, the Los Angeles County Cardiovascular Research Laboratory, University of California and Los Angeles Center for the Health Sciences, Los Angeles, California

Received: June 21 1968

Revision Received: December 12 1968

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1969

J Cell Biol (1969) 41 (2): 401–423.

https://doi.org/10.1083/jcb.41.2.401

This study was designed to investigate the proposition that subcellular calcium is sequestered in specific sites in mammalian myocardium. 29 functioning dog papillary muscles were fixed through the intact vascular supply by means of osmium tetroxide containing a 2% concentration of potassium pyroantimonate (K₂H₂Sb₂O₇·₄H₂O). Tissue examined in the electron microscope showed a consistent and reproducible localization of the electron-opaque pyroantimonate salts of sodium and calcium to distinct sites in the tissue. Sodium pyroantimonate was found exclusively in the extracellular space and clustered at the sarcolemmal membrane. Calcium pyroantimonate, on the other hand, identified primarily by its susceptibility to removal by chelation with EGTA and EDTA, was consistently found densely concentrated in the lateral sacs of the sarcoplasmic reticulum and over the sarcomeric I bands. M zones were virtually free of precipitate. The implications of these findings with respect to various parameters of muscle function are discussed.

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