EXPANSION OF THE INNER MEMBRANE COMPARTMENT AND ITS RELATION TO MITOCHONDRIAL VOLUME AND ION TRANSPORT

Packer , L.; Wrigglesworth , J. M.; Fortes , P. A. G.; Pressman , B. C

doi:10.1083/jcb.39.2.382

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Article| November 01 1968

EXPANSION OF THE INNER MEMBRANE COMPARTMENT AND ITS RELATION TO MITOCHONDRIAL VOLUME AND ION TRANSPORT

L. Packer,

L. Packer

From the Department of Physiology-Anatomy, University of California, Berkeley, California 94720, and Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania

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J. M. Wrigglesworth,

J. M. Wrigglesworth

From the Department of Physiology-Anatomy, University of California, Berkeley, California 94720, and Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania

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P. A. G. Fortes,

P. A. G. Fortes

From the Department of Physiology-Anatomy, University of California, Berkeley, California 94720, and Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania

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B. C Pressman

From the Department of Physiology-Anatomy, University of California, Berkeley, California 94720, and Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania

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Author and Article Information

L. Packer

From the Department of Physiology-Anatomy, University of California, Berkeley, California 94720, and Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania

J. M. Wrigglesworth

From the Department of Physiology-Anatomy, University of California, Berkeley, California 94720, and Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania

P. A. G. Fortes

From the Department of Physiology-Anatomy, University of California, Berkeley, California 94720, and Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania

B. C Pressman

From the Department of Physiology-Anatomy, University of California, Berkeley, California 94720, and Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania

Received: May 10 1968

Revision Received: July 05 1968

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1968

J Cell Biol (1968) 39 (2): 382–391.

https://doi.org/10.1083/jcb.39.2.382

Glutaraldehyde has been used to fix mitochondria undergoing rapid volume changes associated with energized ion transport under oscillatory state conditions and valinomycin-induced potassium uptake. Fixation was found to prevent structural changes which normally occur during ion accumulation or loss. By correlating packed volume measurements with electron microscopy, it is shown that changes in volume associated with ion movements reflect changes in the inner membrane compartment and that this compartment can be related to the sucrose inaccessible space. The method can therefore be used to accurately determine volume changes that arise from ion translocation.

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EXPANSION OF THE INNER MEMBRANE COMPARTMENT AND ITS RELATION TO MITOCHONDRIAL VOLUME AND ION TRANSPORT

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