Cilia were isolated from Tetrahymena pyriformis by an ethanol-calcium procedure. Solutions of outer-fiber protein were obtained either by aqueous extraction of an acetone powder of whole cilia, or by dissolving the isolated outer-fibers in 0.6 M KCl. In aqueous solution, the outer-fiber protein has a sedimentation coefficient of 6.0S and a molecular weight of 104,000 ± 14,000. In 5 M guanidine hydrochloride solution the molecular weight falls to 55,000 ± 5,000. After reduction and alkylation in 8 M urea, about 95% of the protein migrates as a single band on electrophoresis in polyacrylamide gel at pH 8.9; the migration velocity is identical with that of reduced and alkylated actin. Freshly prepared outer-fiber protein contains about 7.5 sulfhydryl groups per 55,000 g of protein. The amino acid composition of outer-fiber protein resembles that of actin, with such differences as occur being of the same order as those between actins from different species of animal.
Article|
January 01 1968
SOME PROPERTIES OF THE PROTEIN FORMING THE OUTER FIBERS OF CILIA
F. L. Renaud,
F. L. Renaud
From the Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
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A. J. Rowe,
A. J. Rowe
From the Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
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I. R. Gibbons
I. R. Gibbons
From the Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
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F. L. Renaud
From the Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
A. J. Rowe
From the Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
I. R. Gibbons
From the Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
Dr. Renaud's present address is the Department of Biology, Yale University, New Haven, Connecticut 06520. The present address of Dr. Rowe is the Department of Biochemistry, University, of Leicester, Leicester, England. Dr. Gibbons' present address is the Pacific Biomedical Research Center, the University of Hawaii, Honolulu, Hawaii 96822
Received:
July 03 1967
Revision Received:
September 25 1967
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1968 by The Rockefeller University Press
1968
J Cell Biol (1968) 36 (1): 79–90.
Article history
Received:
July 03 1967
Revision Received:
September 25 1967
Citation
F. L. Renaud, A. J. Rowe, I. R. Gibbons; SOME PROPERTIES OF THE PROTEIN FORMING THE OUTER FIBERS OF CILIA . J Cell Biol 1 January 1968; 36 (1): 79–90. doi: https://doi.org/10.1083/jcb.36.1.79
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