Morphological and metabolic observations have been made on the effects of endotoxin, deoxycholate, and digitonin (at less than 50 µg/ml) on polymorphonuclear leukocytes and mononuclear cells. The agents stimulate the respiration and glucose oxidation of these cells in a manner similar to that seen during phagocytosis. Electron microscopy revealed no morphological changes with the first two agents, but dramatic membrane changes were seen in the case of digitonin. Here tubular projections of characteristic size and shape formed on and split off the membrane. All the agents stimulated uptake of inulin, but efforts to demonstrate increased pinocytosis by electron microscopy have not so far succeeded, probably due to limitations in present experimental techniques.
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1 March 1967
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March 01 1967
METABOLIC AND MORPHOLOGICAL OBSERVATIONS ON THE EFFECT OF SURFACE-ACTIVE AGENTS ON LEUKOCYTES
R. C. Graham, Jr.,
R. C. Graham, Jr.
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
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M. J. Karnovsky,
M. J. Karnovsky
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
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A. W. Shafer,
A. W. Shafer
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
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E. A. Glass,
E. A. Glass
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
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Manfred L. Karnovsky
Manfred L. Karnovsky
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
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R. C. Graham, Jr.
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
M. J. Karnovsky
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
A. W. Shafer
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
E. A. Glass
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
Manfred L. Karnovsky
From the Departments of Pathology and Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
Dr. Graham's present address is the Department of Medicine, Western Reserve University, Lakeside Hospital, Cleveland, Ohio
Received:
July 29 1966
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1967 by The Rockefeller University Press
1967
J Cell Biol (1967) 32 (3): 629–647.
Article history
Received:
July 29 1966
Citation
R. C. Graham, M. J. Karnovsky, A. W. Shafer, E. A. Glass, Manfred L. Karnovsky; METABOLIC AND MORPHOLOGICAL OBSERVATIONS ON THE EFFECT OF SURFACE-ACTIVE AGENTS ON LEUKOCYTES . J Cell Biol 1 March 1967; 32 (3): 629–647. doi: https://doi.org/10.1083/jcb.32.3.629
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