FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS

Packer , Lester; Nobel , Park S.; Gross , Elizabeth L.; Mel , Howard C.

doi:10.1083/jcb.28.3.443

Article navigation

Article| March 01 1966

FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS

Lester Packer,

Lester Packer

From the Department of Physiology and the Donner Laboratory, University of California, Berkeley

Search for other works by this author on:

This Site

PubMed

Google Scholar

Park S. Nobel,

Park S. Nobel

From the Department of Physiology and the Donner Laboratory, University of California, Berkeley

Search for other works by this author on:

This Site

PubMed

Google Scholar

Elizabeth L. Gross,

Elizabeth L. Gross

From the Department of Physiology and the Donner Laboratory, University of California, Berkeley

Search for other works by this author on:

This Site

PubMed

Google Scholar

Howard C. Mel

From the Department of Physiology and the Donner Laboratory, University of California, Berkeley

Search for other works by this author on:

This Site

PubMed

Google Scholar

Author and Article Information

Lester Packer

From the Department of Physiology and the Donner Laboratory, University of California, Berkeley

Park S. Nobel

From the Department of Physiology and the Donner Laboratory, University of California, Berkeley

Elizabeth L. Gross

From the Department of Physiology and the Donner Laboratory, University of California, Berkeley

Howard C. Mel

From the Department of Physiology and the Donner Laboratory, University of California, Berkeley

Received: September 14 1965

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1966

J Cell Biol (1966) 28 (3): 443–448.

https://doi.org/10.1083/jcb.28.3.443

A separation of spinach chloroplasts in vitro into fractions according to size (volume) and activity (light-dependent shrinkage and NADP reduction) has been achieved by stable-flow free boundary sedimentation-electrophoresis. The salient features of this chloroplast study are: (a) separation is achieved within 30 min; (b) only small density gradients are required, thus minimizing osmotic effects; (c) the fractions are collected continuously, with size fractionation being evidenced; and (d) particles are separated into fractions of higher and lower activities as compared with the control population.

This content is only available as a PDF.

1966

You do not currently have access to this content.

Don't already have an account? Register

You could not be signed in. Please check your email address / username and password and try again.

FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS

Sign in

Client Account

Sign in via your Institution

Suggested Content

Email alerts

This Feature Is Available To Subscribers Only