Six intracellular hydrolases known to be associated with lysosomes in rat liver were found in rat intestinal mucosa. The extent to which they were particulate-bound and the degree of enzyme release when the particulate fractions were suspended in hypotonic media followed the same pattern in both mucosa and liver. The specific activities of the mucosa enzymes were either comparable to or slightly smaller than those of the liver enzymes. These results suggest that the mucosa hydrolases belong to lysosome-like particles. However, differential fractionation of the mucosa indicated that the particles from the mucosa sediment at lower centrifugal forces than do those from the liver and are more heterogeneous in size, bearing a closer resemblance to kidney lysosomes. Possible physiological functions of particulate-bound digestive enzymes in intestinal mucosa are discussed.
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1 November 1964
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November 01 1964
LYSOSOMAL ENZYMES OF RAT INTESTINAL MUCOSA
Lichu Hsu,
Lichu Hsu
From the Department of Food Science and Technology, University of California, Davis, California.
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A. L. Tappel
A. L. Tappel
From the Department of Food Science and Technology, University of California, Davis, California.
Search for other works by this author on:
Lichu Hsu
From the Department of Food Science and Technology, University of California, Davis, California.
A. L. Tappel
From the Department of Food Science and Technology, University of California, Davis, California.
Dr. Hsu's present address is Department of Biochemistry, University of California School of Medicine, San Francisco
Received:
January 14 1964
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1964 by The Rockefeller Institute Press
1964
J Cell Biol (1964) 23 (2): 233–240.
Article history
Received:
January 14 1964
Citation
Lichu Hsu, A. L. Tappel; LYSOSOMAL ENZYMES OF RAT INTESTINAL MUCOSA . J Cell Biol 1 November 1964; 23 (2): 233–240. doi: https://doi.org/10.1083/jcb.23.2.233
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