A repair protein (green) that lacks its kinase activity (left) or phosphorylation sites (right) still turns up at the repair site (blue).

ADNA repair protein turns up at the job site even without its tool kit, according to Uematsu et al. (page 219). Its visit is then prolonged by its inefficiency.

Fixing double strand breaks in DNA by nonhomologous end joining (NHEJ) requires a DNA-dependent protein kinase (DNA-PK) to bind to the loose ends of broken DNA and a ligase to do the gluing. This protein machinery must turn up and fix the ends rapidly to minimize the chance that DNA diffusion causes the wrong partners to be glued back together.

Very little is known, however, about the in vivo dynamics of NHEJ. Here, Uematsu et al. describe the dynamics of DNA–PK recruitment in vivo. DNA-PK is composed of two subunits: Ku70/80 and DNA-PK...

The Rockefeller University Press
2007
The Rockefeller University Press
2007
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