Amajor function of the endocytic system is the sorting of cargo to various organelles. Endocytic sorting of the yeast reductive iron transporter, which is composed of the Fet3 and Ftr1 proteins, is regulated by available iron. When iron is provided to iron-starved cells, Fet3p–Ftr1p is targeted to the lysosome-like vacuole and degraded. In contrast, when iron is not available, Fet3p–Ftr1p is maintained on the plasma membrane via an endocytic recycling pathway requiring the sorting nexin Grd19/Snx3p, the pentameric retromer complex, and the Ypt6p Golgi Rab GTPase module. A recycling signal in Ftr1p was identified and found to bind directly to Grd19/Snx3p. Retromer and Grd19/Snx3p partially colocalize to tubular endosomes, where they are physically associated. After export from the endosome, Fet3p–Ftr1p transits through the Golgi apparatus for resecretion. Thus, Grd19/Snx3p, functions as a cargo-specific adapter for the retromer complex, establishing a precedent for a mechanism by which sorting nexins expand the repertoire of retromer-dependent cargos.
Skip Nav Destination
Article navigation
9 April 2007
Article|
April 09 2007
Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling
Todd I. Strochlic,
Todd I. Strochlic
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Search for other works by this author on:
Thanuja Gangi Setty,
Thanuja Gangi Setty
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Search for other works by this author on:
Anand Sitaram,
Anand Sitaram
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Search for other works by this author on:
Christopher G. Burd
Christopher G. Burd
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Search for other works by this author on:
Todd I. Strochlic
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Thanuja Gangi Setty
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Anand Sitaram
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Christopher G. Burd
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Correspondence to Christopher G. Burd: [email protected]
Abbreviations used in this paper: BAR, Bin/Amphiphysin/Rvs; CPY, carboxypeptidase Y; DSP, dithiobis succinimidyl proprionate; ECF, enhanced chemifluorescence; PtdIns(3)P, phosphatidylinositol-3-phosphate; PX, phox homology.
Received:
September 26 2006
Accepted:
March 12 2007
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2007
J Cell Biol (2007) 177 (1): 115–125.
Article history
Received:
September 26 2006
Accepted:
March 12 2007
Citation
Todd I. Strochlic, Thanuja Gangi Setty, Anand Sitaram, Christopher G. Burd; Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling . J Cell Biol 9 April 2007; 177 (1): 115–125. doi: https://doi.org/10.1083/jcb.200609161
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
Advertisement