In budding yeast, two classes of post-Golgi secretory vesicles carrying different sets of cargoes typified by Bgl2p and invertase are delivered to the plasma membrane for secretion. The exocyst is implicated in tethering these vesicles to the daughter cell membrane for exocytosis. In this study, we report that mutations in the exocyst component Exo70p predominantly block secretion of the Bgl2p vesicles. Furthermore, a defect in invertase vesicle trafficking caused by vps1Δ or pep12Δ in the exo70 mutant background is detrimental to the cell. The secretion defect in exo70 mutants was most pronounced during the early budding stage, which affected daughter cell growth. The selective secretion block does not occur at the vesicle formation or sorting stage because the exocytic vesicles are properly generated and protein processing is normal in the exo70 mutants. Our study suggests that Exo70p functions primarily at early stages of the cell cycle in Bgl2p vesicle secretion, which is critical for polarized cell growth.
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12 March 2007
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March 05 2007
Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth
Bing He,
Bing He
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
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Fengong Xi,
Fengong Xi
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
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Jian Zhang,
Jian Zhang
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
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Daniel TerBush,
Daniel TerBush
2Department of Biochemistry, Uniformed Services University of Health Sciences, Bethesda, MD 20814
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Xiaoyu Zhang,
Xiaoyu Zhang
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
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Wei Guo
Wei Guo
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
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Bing He
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
Fengong Xi
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
Jian Zhang
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
Daniel TerBush
2Department of Biochemistry, Uniformed Services University of Health Sciences, Bethesda, MD 20814
Xiaoyu Zhang
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
Wei Guo
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
Correspondence to Wei Guo: [email protected]
B. He and F. Xi contributed equally to this paper.
Abbreviations used in this paper: 5-FOA, 5-fluoroorotic acid; CPY, carboxypeptidase Y; SC, synthetic complete media; YPD, yeast extract/peptone/glucose.
Received:
June 27 2006
Accepted:
February 01 2007
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2007
J Cell Biol (2007) 176 (6): 771–777.
Article history
Received:
June 27 2006
Accepted:
February 01 2007
Citation
Bing He, Fengong Xi, Jian Zhang, Daniel TerBush, Xiaoyu Zhang, Wei Guo; Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth . J Cell Biol 12 March 2007; 176 (6): 771–777. doi: https://doi.org/10.1083/jcb.200606134
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