Three-dimensional images of the undercoat structure on the cytoplasmic surface of the upper cell membrane of normal rat kidney fibroblast (NRK) cells and fetal rat skin keratinocytes were reconstructed by electron tomography, with 0.85-nm–thick consecutive sections made ∼100 nm from the cytoplasmic surface using rapidly frozen, deeply etched, platinum-replicated plasma membranes. The membrane skeleton (MSK) primarily consists of actin filaments and associated proteins. The MSK covers the entire cytoplasmic surface and is closely linked to clathrin-coated pits and caveolae. The actin filaments that are closely apposed to the cytoplasmic surface of the plasma membrane (within 10.2 nm) are likely to form the boundaries of the membrane compartments responsible for the temporary confinement of membrane molecules, thus partitioning the plasma membrane with regard to their lateral diffusion. The distribution of the MSK mesh size as determined by electron tomography and that of the compartment size as determined from high speed single-particle tracking of phospholipid diffusion agree well in both cell types, supporting the MSK fence and MSK-anchored protein picket models.
Skip Nav Destination
Article navigation
11 September 2006
Article|
September 05 2006
Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography
Nobuhiro Morone,
Nobuhiro Morone
1Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Nagoya 460-0012, Japan
2Department of Cell Biology and Anatomy, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan
3Department of Ultrastructural Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan
Search for other works by this author on:
Takahiro Fujiwara,
Takahiro Fujiwara
4Membrane Mechanisms Project, International Cooperative Research Project (ICORP), Japan Science and Technology Agency, Institute for Frontier Medical Sciences, Kyoto University, Shougoin, Kyoto 606-8507, Japan
Search for other works by this author on:
Kotono Murase,
Kotono Murase
1Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Nagoya 460-0012, Japan
Search for other works by this author on:
Rinshi S. Kasai,
Rinshi S. Kasai
4Membrane Mechanisms Project, International Cooperative Research Project (ICORP), Japan Science and Technology Agency, Institute for Frontier Medical Sciences, Kyoto University, Shougoin, Kyoto 606-8507, Japan
Search for other works by this author on:
Hiroshi Ike,
Hiroshi Ike
1Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Nagoya 460-0012, Japan
Search for other works by this author on:
Shigeki Yuasa,
Shigeki Yuasa
3Department of Ultrastructural Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan
Search for other works by this author on:
Jiro Usukura,
Jiro Usukura
2Department of Cell Biology and Anatomy, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan
Search for other works by this author on:
Akihiro Kusumi
Akihiro Kusumi
1Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Nagoya 460-0012, Japan
4Membrane Mechanisms Project, International Cooperative Research Project (ICORP), Japan Science and Technology Agency, Institute for Frontier Medical Sciences, Kyoto University, Shougoin, Kyoto 606-8507, Japan
Search for other works by this author on:
Nobuhiro Morone
1Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Nagoya 460-0012, Japan
2Department of Cell Biology and Anatomy, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan
3Department of Ultrastructural Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan
Takahiro Fujiwara
4Membrane Mechanisms Project, International Cooperative Research Project (ICORP), Japan Science and Technology Agency, Institute for Frontier Medical Sciences, Kyoto University, Shougoin, Kyoto 606-8507, Japan
Kotono Murase
1Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Nagoya 460-0012, Japan
Rinshi S. Kasai
4Membrane Mechanisms Project, International Cooperative Research Project (ICORP), Japan Science and Technology Agency, Institute for Frontier Medical Sciences, Kyoto University, Shougoin, Kyoto 606-8507, Japan
Hiroshi Ike
1Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Nagoya 460-0012, Japan
Shigeki Yuasa
3Department of Ultrastructural Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan
Jiro Usukura
2Department of Cell Biology and Anatomy, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan
Akihiro Kusumi
1Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Nagoya 460-0012, Japan
4Membrane Mechanisms Project, International Cooperative Research Project (ICORP), Japan Science and Technology Agency, Institute for Frontier Medical Sciences, Kyoto University, Shougoin, Kyoto 606-8507, Japan
Correspondence to Akihiro Kusumi: [email protected]
Abbreviations used in this paper: CCP, clathrin-coated pit; FRSK, fetal rat skin keratinocyte; MSK, membrane skeleton; NRK, normal rat kidney fibroblast.
Received:
June 02 2006
Accepted:
August 04 2006
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2006
J Cell Biol (2006) 174 (6): 851–862.
Article history
Received:
June 02 2006
Accepted:
August 04 2006
Connected Content
Related
Sizing up actin fences
Citation
Nobuhiro Morone, Takahiro Fujiwara, Kotono Murase, Rinshi S. Kasai, Hiroshi Ike, Shigeki Yuasa, Jiro Usukura, Akihiro Kusumi; Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography . J Cell Biol 11 September 2006; 174 (6): 851–862. doi: https://doi.org/10.1083/jcb.200606007
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
See also
Email alerts
Advertisement
Advertisement