Caught in the act: EM-reconstructed stereo pair of a lateral microtubule capturing a kinetochore.

RIEDER

With the rise of VHS in the 1980s came routine video time-lapse imaging of cellular phenomena in living cells. One of the first to take advantage of the new technique was Conly Rieder at the Wadsworth Center in Albany, NY.

Rieder was probing the origin of the microtubules that make up the kinetochore fibers (K-fibers), which attach chromosomes to the spindle during early prometaphase. In vitro data supported both that kinetochores trapped microtubules (MTs) growing from the pole and that kinetochores could nucleate microtubules directly (Mitchison and Kirschner, 1985a,b).

In 1981, Rieder and Gary Borisy had shown that during recovery from cold treatment, kinetochores did not nucleate MTs, and that those closest to the centrosomes attached first (Rieder and Borisy, 1981). But such in...

The Rockefeller University Press
2006
The Rockefeller University Press
2006
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