Cell determination in the retina has been under intense investigation since the discovery that retinal progenitors generate clones of apparently random composition (Price, J., D. Turner, and C. Cepko. 1987. Proc. Natl. Acad. Sci. USA. 84:156–160; Holt, C.E., T.W. Bertsch, H.M. Ellis, and W.A. Harris. 1988. Neuron. 1:15–26; Wetts, R., and S.E. Fraser. 1988. Science. 239:1142–1145). Examination of fixed tissue, however, sheds little light on lineage patterns or on the relationship between the orientation of division and cell fate. In this study, three-dimensional time-lapse analyses were used to trace lineages of retinal progenitors expressing green fluorescent protein under the control of the ath5 promoter. Surprisingly, these cells divide just once along the circumferential axis to produce two postmitotic daughters, one of which becomes a retinal ganglion cell (RGC). Interestingly, when these same progenitors are transplanted into a mutant environment lacking RGCs, they often divide along the central-peripheral axis and produce two RGCs. This study provides the first insight into reproducible lineage patterns of retinal progenitors in vivo and the first evidence that environmental signals influence the orientation of cell division and the lineage of neural progenitors.
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19 December 2005
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December 19 2005
Influences on neural lineage and mode of division in the zebrafish retina in vivo
Lucia Poggi,
Lucia Poggi
1Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, United Kingdom
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Marta Vitorino,
Marta Vitorino
1Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, United Kingdom
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Ichiro Masai,
Ichiro Masai
2RIKEN Institute of Physical and Chemical Research, Hirosawa, Saitama 351-0198, Japan
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William A. Harris
William A. Harris
1Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, United Kingdom
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Lucia Poggi
1Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, United Kingdom
Marta Vitorino
1Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, United Kingdom
Ichiro Masai
2RIKEN Institute of Physical and Chemical Research, Hirosawa, Saitama 351-0198, Japan
William A. Harris
1Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, United Kingdom
Correspondence to William A. Harris: [email protected]
Abbreviations used in this paper: 3D, three dimensional; GAP, growth-associated protein; GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; RGC, retinal ganglion cell.
Received:
September 16 2005
Accepted:
November 14 2005
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2005
J Cell Biol (2005) 171 (6): 991–999.
Article history
Received:
September 16 2005
Accepted:
November 14 2005
Citation
Lucia Poggi, Marta Vitorino, Ichiro Masai, William A. Harris; Influences on neural lineage and mode of division in the zebrafish retina in vivo . J Cell Biol 19 December 2005; 171 (6): 991–999. doi: https://doi.org/10.1083/jcb.200509098
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