Rat liver nuclei, after preliminary isolation in 2.2 molar sucrose solution, were separated into density classes by centrifugation at 95,000 g for 45 to 85 minutes in a sucrose density gradient (density range, 1.28 to 1.33). Nuclei from normal liver separated into three bands with average DNA phosphorus content per nucleus of 0.67, 0.84, and 0.93 picogram for top, middle, and bottom bands, respectively. Nuclei from regenerating liver (26 hours after one-third hepatectomy) yielded three bands and a pellet fraction with average DNA phosphorus content per nucleus of 0.76, 1.02, 1.38, and 1.51 picograms (top to bottom of tube). This method appears capable of yielding nuclei which have increased their DNA content prior to mitosis, and this procedure should be valuable in studies of biochemical changes which occur in nuclei preparing for mitosis.
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1 May 1963
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May 01 1963
DENSITY GRADIENT ISOLATION OF RAT LIVER NUCLEI WITH HIGH DNA CONTENT
Richard F. Fisher,
Richard F. Fisher
From the Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill
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David J. Holbrook, Jr.,
David J. Holbrook, Jr.
From the Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill
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J. Logan Irvin
J. Logan Irvin
From the Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill
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Richard F. Fisher
From the Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill
David J. Holbrook, Jr.
From the Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill
J. Logan Irvin
From the Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill
Received:
July 27 1962
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright, 1963, by The Rockefeller Institute Press
1963
J Cell Biol (1963) 17 (2): 231–236.
Article history
Received:
July 27 1962
Citation
Richard F. Fisher, David J. Holbrook, J. Logan Irvin; DENSITY GRADIENT ISOLATION OF RAT LIVER NUCLEI WITH HIGH DNA CONTENT . J Cell Biol 1 May 1963; 17 (2): 231–236. doi: https://doi.org/10.1083/jcb.17.2.231
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