Musculin/MyoR is a new member of basic helix-loop-helix transcription factors, and its expression is limited to skeletal muscle precursors. Here, we report that musculin/MyoR is expressed in adult kidney side population (SP) cells and can regulate their function. SP phenotype can be used to purify stem cell–rich fractions. Microarray analysis clarified that musculin/MyoR was exclusively expressed in kidney SP cells, and the cells resided in the renal interstitial space. Musculin/MyoR-positive cells were decreased in acute renal failure, but infusion of kidney SP cells increased musculin/MyoR-positive cells and improved renal function. Kidney SP cells in reversible acute renal failure expressed a high level of renoprotective factors and leukemia inhibitory factor (LIF), but not in irreversible chronic renal failure. In cultured kidney SP cells, LIF stimulated gene expression of renoprotective factors, and down-regulation of musculin/MyoR augmented LIF-induced gene expression. Our results suggest that musculin/MyoR may play important roles not only in developmental processes but also in regenerative processes in adult tissue.
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20 June 2005
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June 20 2005
Musculin/MyoR is expressed in kidney side population cells and can regulate their function
Keiichi Hishikawa,
Keiichi Hishikawa
1Department of Clinical Renal Regeneration, Graduate School of Medicine
2Department of Internal Medicine, Division of Nephrology and Endocrinology
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Takeshi Marumo,
Takeshi Marumo
1Department of Clinical Renal Regeneration, Graduate School of Medicine
2Department of Internal Medicine, Division of Nephrology and Endocrinology
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Shigeki Miura,
Shigeki Miura
1Department of Clinical Renal Regeneration, Graduate School of Medicine
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Asato Nakanishi,
Asato Nakanishi
1Department of Clinical Renal Regeneration, Graduate School of Medicine
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Yumi Matsuzaki,
Yumi Matsuzaki
4Department of Physiology, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
9Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo 102-8666, Japan
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Katsunori Shibata,
Katsunori Shibata
1Department of Clinical Renal Regeneration, Graduate School of Medicine
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Tomoko Ichiyanagi,
Tomoko Ichiyanagi
1Department of Clinical Renal Regeneration, Graduate School of Medicine
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Hiroko Kohike,
Hiroko Kohike
4Department of Physiology, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
9Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo 102-8666, Japan
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Takuya Komori,
Takuya Komori
4Department of Physiology, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
9Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo 102-8666, Japan
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Ichiro Takahashi,
Ichiro Takahashi
6Central Electron Microscopic Laboratory, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, Tokyo 173, Japan
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Osamu Takase,
Osamu Takase
5Department of Internal Medicine, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
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Naohiko Imai,
Naohiko Imai
5Department of Internal Medicine, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
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Masahiro Yoshikawa,
Masahiro Yoshikawa
1Department of Clinical Renal Regeneration, Graduate School of Medicine
2Department of Internal Medicine, Division of Nephrology and Endocrinology
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Toshihiko Inowa,
Toshihiko Inowa
1Department of Clinical Renal Regeneration, Graduate School of Medicine
3Department of Urology, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan
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Matsuhiko Hayashi,
Matsuhiko Hayashi
5Department of Internal Medicine, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
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Toshio Nakaki,
Toshio Nakaki
7Department of Pharmacology, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, Tokyo 173, Japan
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Hiromitsu Nakauchi,
Hiromitsu Nakauchi
8The Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo 108, Japan
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Hideyuki Okano,
Hideyuki Okano
4Department of Physiology, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
9Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo 102-8666, Japan
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Toshiro Fujita
Toshiro Fujita
1Department of Clinical Renal Regeneration, Graduate School of Medicine
2Department of Internal Medicine, Division of Nephrology and Endocrinology
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Keiichi Hishikawa
1Department of Clinical Renal Regeneration, Graduate School of Medicine
2Department of Internal Medicine, Division of Nephrology and Endocrinology
Takeshi Marumo
1Department of Clinical Renal Regeneration, Graduate School of Medicine
2Department of Internal Medicine, Division of Nephrology and Endocrinology
Shigeki Miura
1Department of Clinical Renal Regeneration, Graduate School of Medicine
Asato Nakanishi
1Department of Clinical Renal Regeneration, Graduate School of Medicine
Yumi Matsuzaki
4Department of Physiology, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
9Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo 102-8666, Japan
Katsunori Shibata
1Department of Clinical Renal Regeneration, Graduate School of Medicine
Tomoko Ichiyanagi
1Department of Clinical Renal Regeneration, Graduate School of Medicine
Hiroko Kohike
4Department of Physiology, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
9Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo 102-8666, Japan
Takuya Komori
4Department of Physiology, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
9Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo 102-8666, Japan
Ichiro Takahashi
6Central Electron Microscopic Laboratory, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, Tokyo 173, Japan
Osamu Takase
5Department of Internal Medicine, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
Naohiko Imai
5Department of Internal Medicine, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
Masahiro Yoshikawa
1Department of Clinical Renal Regeneration, Graduate School of Medicine
2Department of Internal Medicine, Division of Nephrology and Endocrinology
Toshihiko Inowa
1Department of Clinical Renal Regeneration, Graduate School of Medicine
3Department of Urology, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan
Matsuhiko Hayashi
5Department of Internal Medicine, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
Toshio Nakaki
7Department of Pharmacology, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, Tokyo 173, Japan
Hiromitsu Nakauchi
8The Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo 108, Japan
Hideyuki Okano
4Department of Physiology, Keio University School of Medicine, Shionanomachi 35, Shinjuku-ku, Tokyo 160, Japan
9Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo 102-8666, Japan
Toshiro Fujita
1Department of Clinical Renal Regeneration, Graduate School of Medicine
2Department of Internal Medicine, Division of Nephrology and Endocrinology
Correspondence to Keiichi Hishikawa: [email protected]
Abbreviations used in this paper: ABC, ATP-binding cassette; ARF, acute renal failure; bHLH, basic helix-loop-helix; BM, bone marrow-derived mononuclear; BMP, bone morphologic protein; BUN, blood urea nitrogen; CRF, chronic renal failure; HGF, hepatocyte growth factor; LIF, leukemia inhibitory factor; RA, retinoic acid; SP, side population; TSA, trichostatin A.
Received:
December 29 2004
Accepted:
May 16 2005
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2005
J Cell Biol (2005) 169 (6): 921–928.
Article history
Received:
December 29 2004
Accepted:
May 16 2005
Citation
Keiichi Hishikawa, Takeshi Marumo, Shigeki Miura, Asato Nakanishi, Yumi Matsuzaki, Katsunori Shibata, Tomoko Ichiyanagi, Hiroko Kohike, Takuya Komori, Ichiro Takahashi, Osamu Takase, Naohiko Imai, Masahiro Yoshikawa, Toshihiko Inowa, Matsuhiko Hayashi, Toshio Nakaki, Hiromitsu Nakauchi, Hideyuki Okano, Toshiro Fujita; Musculin/MyoR is expressed in kidney side population cells and can regulate their function . J Cell Biol 20 June 2005; 169 (6): 921–928. doi: https://doi.org/10.1083/jcb.200412167
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