In Xenopus oocytes, the spindle assembly checkpoint (SAC) kinase Bub1 is required for cytostatic factor (CSF)-induced metaphase arrest in meiosis II. To investigate whether matured mouse oocytes are kept in metaphase by a SAC-mediated inhibition of the anaphase-promoting complex/cyclosome (APC/C) complex, we injected a dominant-negative Bub1 mutant (Bub1dn) into mouse oocytes undergoing meiosis in vitro. Passage through meiosis I was accelerated, but even though the SAC was disrupted, injected oocytes still arrested at metaphase II. Bub1dn-injected oocytes released from CSF and treated with nocodazole to disrupt the second meiotic spindle proceeded into interphase, whereas noninjected control oocytes remained arrested at metaphase. Similar results were obtained using dominant-negative forms of Mad2 and BubR1, as well as checkpoint resistant dominant APC/C activating forms of Cdc20. Thus, SAC proteins are required for checkpoint functions in meiosis I and II, but, in contrast to frog eggs, the SAC is not required for establishing or maintaining the CSF arrest in mouse oocytes.
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20 December 2004
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December 20 2004
The spindle assembly checkpoint is not essential for CSF arrest of mouse oocytes
Chizuko Tsurumi,
Chizuko Tsurumi
1Max-Planck-Institut fuer Immunbiologie Developmental Biology, 79108 Freiburg, Germany
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Steffen Hoffmann,
Steffen Hoffmann
1Max-Planck-Institut fuer Immunbiologie Developmental Biology, 79108 Freiburg, Germany
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Stephan Geley,
Stephan Geley
2Institute of Pathophysiology, Medical University of Innsbruck, A-6020 Innsbruck, Austria
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Ralph Graeser,
Ralph Graeser
3ProQinase GMBH, 79106 Freiburg, Germany
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Zbigniew Polanski
Zbigniew Polanski
1Max-Planck-Institut fuer Immunbiologie Developmental Biology, 79108 Freiburg, Germany
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Chizuko Tsurumi
1Max-Planck-Institut fuer Immunbiologie Developmental Biology, 79108 Freiburg, Germany
Steffen Hoffmann
1Max-Planck-Institut fuer Immunbiologie Developmental Biology, 79108 Freiburg, Germany
Stephan Geley
2Institute of Pathophysiology, Medical University of Innsbruck, A-6020 Innsbruck, Austria
Ralph Graeser
3ProQinase GMBH, 79106 Freiburg, Germany
Zbigniew Polanski
1Max-Planck-Institut fuer Immunbiologie Developmental Biology, 79108 Freiburg, Germany
Correspondence to Chizuko Tsurumi: [email protected]
Abbreviations used in this paper: APC/C, anaphase-promoting complex/cyclosome; CSF, cytostatic factor; GV, germinal vesicle; GVBD, GV breakdown; MPF, maturation promoting factor; PB, polar body; PI, propidium iodide; SAC, spindle assembly checkpoint.
Received:
May 27 2004
Accepted:
November 05 2004
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2004
J Cell Biol (2004) 167 (6): 1037–1050.
Article history
Received:
May 27 2004
Accepted:
November 05 2004
Citation
Chizuko Tsurumi, Steffen Hoffmann, Stephan Geley, Ralph Graeser, Zbigniew Polanski; The spindle assembly checkpoint is not essential for CSF arrest of mouse oocytes . J Cell Biol 20 December 2004; 167 (6): 1037–1050. doi: https://doi.org/10.1083/jcb.200405165
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