Death DNase tied down

An apoptotic cell can cause problems from the grave, including autoimmune disorders, if its DNA is not properly chopped up before death. In human cells, most of this degradation is done by caspase-activated DNase (CAD). On page 851, Lechardeur et al. show that CAD is given free range in the nucleus early in apoptosis, but later gets hitched up—perhaps to prevent it from wreaking havoc outside the cell.

CAD dynamics in living cells indicated that, before apoptosis, CAD and its cobound inhibitor diffused freely in the nucleus. After caspase-3 cleavage of CAD released its inhibitor, CAD continued to move unfettered even as it cleaved DNA. But in time, CAD movements became progressively restricted. Within several hours, after DNA ladders formed, most of the CAD molecules were immobilized.

By this time,...