Single-layered epithelia are the first differentiated cell types to develop in the embryo, with columnar and squamous types appearing immediately after blastocyst implantation. Here, we show that mouse embryonic stem cells seeded on hensin or laminin, but not fibronectin or collagen type IV, formed hemispheric epithelial structures whose outermost layer terminally differentiated to an epithelium that resembled the visceral endoderm. Hensin induced columnar epithelia, whereas laminin formed squamous epithelia. At the egg cylinder stage, the distal visceral endoderm is columnar, and these cells begin to migrate anteriorly to create the anterior visceral endoderm, which assumes a squamous shape. Hensin expression coincided with the dynamic appearance and disappearance of columnar cells at the egg cylinder stage of the embryo. These expression patterns, and the fact that hensin null embryos (and those already reported for laminin) die at the onset of egg cylinder formation, support the view that hensin and laminin are required for terminal differentiation of columnar and squamous epithelial phenotypes during early embryogenesis.
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27 September 2004
Article|
September 27 2004
Conversion of ES cells to columnar epithelia by hensin and to squamous epithelia by laminin
Jiro Takito,
Jiro Takito
Department of Medicine and Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032
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Qais Al-Awqati
Qais Al-Awqati
Department of Medicine and Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032
Search for other works by this author on:
Jiro Takito
Department of Medicine and Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032
Qais Al-Awqati
Department of Medicine and Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032
Address correspondence to Qais Al-Awqati, Dept. of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th St., New York, NY 10032. Tel.: (212) 305-3512. Fax: (212) 305-3475. email: [email protected]
Abbreviations used in this paper: AVE, anterior visceral endoderm; E, embryonic day; ES, embryonic stem; ICM, inner cell mass; LIF, leukemia inhibitory factor.
Received:
May 27 2004
Accepted:
August 16 2004
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2004
J Cell Biol (2004) 166 (7): 1093–1102.
Article history
Received:
May 27 2004
Accepted:
August 16 2004
Citation
Jiro Takito, Qais Al-Awqati; Conversion of ES cells to columnar epithelia by hensin and to squamous epithelia by laminin . J Cell Biol 27 September 2004; 166 (7): 1093–1102. doi: https://doi.org/10.1083/jcb.200405159
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