Preventing licensing with geminin also prevents loading of Scc2 and cohesin.

Hirano/Elsevier

Replication of DNA and cohesion of the resultant sister chromatids are two activities that must be coordinated. Now, Peter Gillespie and Tatsuya Hirano (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) have found that the same “licensing” process that gets DNA ready for replication is also necessary to allow loading of the cohesin complex responsible for sister chromatid cohesion.

Gillespie and Hirano used the sequence of yeast cohesin-loading proteins to identify human and frog versions. They confirmed biochemically in frog extracts what had been inferred genetically in yeast: that Scc2 (in frogs via two isoforms) is required for the loading of cohesin onto DNA.

Association of Scc2 with chromatin was inhibited by two treatments that block DNA replication licensing: addition of geminin, a small protein that binds to the prereplication complex protein Cdt1,...

The Rockefeller University Press
2004
The Rockefeller University Press
2004
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