Dead cells bind dye nonspecifically.

Suicide in a unicellular organism might sound like a lousy idea, but there are several possible justifications. Budding yeast cells that are sickly, mating-incompetent, or stuck in the middle of a colony might do better to go out with a messy bang, thus releasing all their nutrients for their nearby relatives, than to fade away with energy sources locked behind a cell wall.

Yeast do die of causes other than old age, but it remains unclear whether this death is like mammalian apoptosis, and to what extent it is controlled rather than a direct result of an insult to the cell. Apoptosis was put forward as a possibility when a caspase-like protein, Yca1p, was discovered, and dying cells were found to bind a caspase substrate.

But on page 311, Wysocki and Kron bring these conclusions, and the whole concept...

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