Remodelling of the plasma membrane cytoarchitecture is crucial for the regulation of epithelial cell adhesion and permeability. In Madin-Darby canine kidney cells, the protein AHNAK relocates from the cytosol to the cytosolic surface of the plasma membrane during the formation of cell–cell contacts and the development of epithelial polarity. This targeting is reversible and regulated by Ca2+-dependent cell–cell adhesion. At the plasma membrane, AHNAK associates as a multimeric complex with actin and the annexin 2/S100A10 complex. The S100A10 subunit serves to mediate the interaction between annexin 2 and the COOH-terminal regulatory domain of AHNAK. Down-regulation of both annexin 2 and S100A10 using an annexin 2–specific small interfering RNA inhibits the association of AHNAK with plasma membrane. In Madin-Darby canine kidney cells, down-regulation of AHNAK using AHNAK-specific small interfering RNA prevents cortical actin cytoskeleton reorganization required to support cell height. We propose that the interaction of AHNAK with the annexin 2/S100A10 regulates cortical actin cytoskeleton organization and cell membrane cytoarchitecture.
Skip Nav Destination
Article navigation
5 January 2004
Article|
December 29 2003
AHNAK interaction with the annexin 2/S100A10 complex regulates cell membrane cytoarchitecture
Christelle Benaud,
Christelle Benaud
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Search for other works by this author on:
Benoît J. Gentil,
Benoît J. Gentil
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Search for other works by this author on:
Nicole Assard,
Nicole Assard
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Search for other works by this author on:
Magalie Court,
Magalie Court
2Laboratoire de Chimie des Proteines, INSERM ERM-0201, DRDC-CP, CEA-Grenoble, 38054 Grenoble Cedex 9, France
Search for other works by this author on:
Jerome Garin,
Jerome Garin
2Laboratoire de Chimie des Proteines, INSERM ERM-0201, DRDC-CP, CEA-Grenoble, 38054 Grenoble Cedex 9, France
Search for other works by this author on:
Christian Delphin,
Christian Delphin
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Search for other works by this author on:
Jacques Baudier
Jacques Baudier
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Search for other works by this author on:
Christelle Benaud
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Benoît J. Gentil
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Nicole Assard
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Magalie Court
2Laboratoire de Chimie des Proteines, INSERM ERM-0201, DRDC-CP, CEA-Grenoble, 38054 Grenoble Cedex 9, France
Jerome Garin
2Laboratoire de Chimie des Proteines, INSERM ERM-0201, DRDC-CP, CEA-Grenoble, 38054 Grenoble Cedex 9, France
Christian Delphin
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Jacques Baudier
1Laboratoire de Transduction du Signal, INSERM EMI-0104, DRDC-TS
Address correspondence to Jacques Baudier, INSERM EMI-0104, DRDC-TS, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France. Tel.: (33) 4-38-78-43 28. Fax: (33) 4-38-78-50-58. email: [email protected]
The online version of this article includes supplemental material.
Abbreviation used in this paper: siRNA, small interfering RNA.
Received:
July 15 2003
Accepted:
November 20 2003
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2004
J Cell Biol (2004) 164 (1): 133–144.
Article history
Received:
July 15 2003
Accepted:
November 20 2003
Citation
Christelle Benaud, Benoît J. Gentil, Nicole Assard, Magalie Court, Jerome Garin, Christian Delphin, Jacques Baudier; AHNAK interaction with the annexin 2/S100A10 complex regulates cell membrane cytoarchitecture . J Cell Biol 5 January 2004; 164 (1): 133–144. doi: https://doi.org/10.1083/jcb.200307098
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement