We examined global changes in the acetylation of histones in mouse oocytes during meiosis. Immunocytochemistry with specific antibodies against various acetylated lysine residues on histones H3 and H4 showed that acetylation of all the lysines decreased to undetectable or negligible levels in the oocytes during meiosis, whereas most of these lysines were acetylated during mitosis in preimplantation embryos and somatic cells. When the somatic cell nuclei were transferred into enucleated oocytes, the acetylation of lysines decreased markedly. This type of deacetylation was inhibited by trichostatin A, a specific inhibitor of histone deacetylase (HDAC), thereby indicating that HDAC is able to deacetylate histones during meiosis but not during mitosis. Meiosis-specific deacetylation may be a consequence of the accessibility of HDAC1 to the chromosome, because HDAC1 colocalized with the chromosome during meiosis but not during mitosis. As histone acetylation is thought to play a role in propagating the gene expression pattern to the descendent generation during mitosis, and the gene expression pattern of differentiated oocytes is reprogrammed during meiosis to allow the initiation of a new program by totipotent zygotes of the next generation, our results suggest that the oocyte cytoplasm initializes a program of gene expression by deacetylating histones.
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7 July 2003
Article|
June 30 2003
Changes in histone acetylation during mouse oocyte meiosis
Jin-Moon Kim,
Jin-Moon Kim
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
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Honglin Liu,
Honglin Liu
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
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Mayuko Tazaki,
Mayuko Tazaki
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
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Masao Nagata,
Masao Nagata
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
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Fugaku Aoki
Fugaku Aoki
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
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Jin-Moon Kim
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
Honglin Liu
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
Mayuko Tazaki
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
Masao Nagata
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
Fugaku Aoki
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan
Address correspondence to Fugaku Aoki, Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan. Tel.: 81-4-7136-5424. Fax: 81-4-7136-3698. E-mail: [email protected]
J.-M. Kim and H. Liu contributed equally to this work.
The online version of this article includes supplemental material.
*
Abbreviations used in this paper: GV, germinal vesicle; GVBD, germinal vesicle breakdown; HAT, histone acetylase; HDAC, histone deacetylase; IBMX, 3-isobutyl-methylxanthine; MII, metaphase II; TSA, trichostatin A.
Received:
March 07 2003
Revision Received:
May 01 2003
Accepted:
May 20 2003
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2003
J Cell Biol (2003) 162 (1): 37–46.
Article history
Received:
March 07 2003
Revision Received:
May 01 2003
Accepted:
May 20 2003
Citation
Jin-Moon Kim, Honglin Liu, Mayuko Tazaki, Masao Nagata, Fugaku Aoki; Changes in histone acetylation during mouse oocyte meiosis . J Cell Biol 7 July 2003; 162 (1): 37–46. doi: https://doi.org/10.1083/jcb.200303047
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