Podosomes are actin- and fimbrin-containing adhesions at the leading edge of macrophages. In cells transfected with β-actin–ECFP and L-fimbrin–EYFP, quantitative four-dimensional microscopy of podosome assembly shows that new adhesions arise at the cell periphery by one of two mechanisms; de novo podosome assembly, or fission of a precursor podosome into daughter podosomes. The large podosome cluster precursor also appears to be an adhesion structure; it contains actin, fimbrin, integrin, and is in close apposition to the substratum. Microtubule inhibitors paclitaxel and demecolcine inhibit the turnover and polarized formation of podosomes, but not the turnover rate of actin in these structures. Because daughter podosomes and podosome cluster precursors are preferentially located at the leading edge, they may play a critical role in continually generating new sites of cell adhesion.
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26 May 2003
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May 19 2003
Macrophage podosomes assemble at the leading lamella by growth and fragmentation
James G. Evans,
James G. Evans
1BioImaging Center, Cambridge, MA 02142
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
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Ivan Correia,
Ivan Correia
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
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Olga Krasavina,
Olga Krasavina
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
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Nicki Watson,
Nicki Watson
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
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Paul Matsudaira
Paul Matsudaira
1BioImaging Center, Cambridge, MA 02142
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
3Department of Biology and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
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James G. Evans
1BioImaging Center, Cambridge, MA 02142
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
Ivan Correia
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
Olga Krasavina
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
Nicki Watson
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
Paul Matsudaira
1BioImaging Center, Cambridge, MA 02142
2Whitehead Institute for Biomedical Research, Cambridge, MA 02142
3Department of Biology and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
Address correspondence to James G. Evans, Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142. Tel.: (617) 324-0300. Fax: (617) 258-7226. E-mail: [email protected]
The online version of this article includes supplemental material.
*
Abbreviations used in this paper: 2-, 3-, and 4-D, two, three, and four dimensional; IRM, interference reflection microscopy; PCP, podosome cluster precursor.
Received:
December 04 2002
Revision Received:
March 31 2003
Accepted:
April 01 2003
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2003
J Cell Biol (2003) 161 (4): 697–705.
Article history
Received:
December 04 2002
Revision Received:
March 31 2003
Accepted:
April 01 2003
Citation
James G. Evans, Ivan Correia, Olga Krasavina, Nicki Watson, Paul Matsudaira; Macrophage podosomes assemble at the leading lamella by growth and fragmentation . J Cell Biol 26 May 2003; 161 (4): 697–705. doi: https://doi.org/10.1083/jcb.200212037
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